Genetic and physical mapping of flowering time loci in canola (Brassica napus L.)

Raman, Harsh, Raman, Rosy, Eckermann, Paul, Coombes, Neil, Manoli, Sahana, Zou, Xiaoxiao, Edwards, David, Meng, Jinling, Prangnell, Roslyn, Stiller, Jiri, Batley, Jacqueline, Luckett, David, Wratten, Neil and Dennis, Elizabeth (2013) Genetic and physical mapping of flowering time loci in canola (Brassica napus L.). Theoretical and Applied Genetics, 126 1: 119-132. doi:10.1007/s00122-012-1966-8

Author Raman, Harsh
Raman, Rosy
Eckermann, Paul
Coombes, Neil
Manoli, Sahana
Zou, Xiaoxiao
Edwards, David
Meng, Jinling
Prangnell, Roslyn
Stiller, Jiri
Batley, Jacqueline
Luckett, David
Wratten, Neil
Dennis, Elizabeth
Title Genetic and physical mapping of flowering time loci in canola (Brassica napus L.)
Formatted title
Genetic and physical mapping of flowering time loci in canola (Brassica napus L.)
Journal name Theoretical and Applied Genetics   Check publisher's open access policy
ISSN 0040-5752
Publication date 2013-01
Year available 2012
Sub-type Article (original research)
DOI 10.1007/s00122-012-1966-8
Volume 126
Issue 1
Start page 119
End page 132
Total pages 14
Place of publication Heidelberg, Germany
Publisher Springer
Collection year 2013
Language eng
Formatted abstract
We identified quantitative trait loci (QTL) underlying variation for flowering time in a doubled haploid (DH) population of vernalisation-responsive canola (Brassica napus L.) cultivars Skipton and Ag-Spectrum and aligned them with physical map positions of predicted flowering genes from the Brassica rapa genome. Significant genetic variation in flowering time and response to vernalisation were observed among the DH lines from Skipton/Ag-Spectrum. A molecular linkage map was generated comprising 674 simple sequence repeat, sequence-related amplified polymorphism, sequence characterised amplified region, Diversity Array Technology, and candidate gene based markers loci. QTL analysis indicated that flowering time is a complex trait and is controlled by at least 20 loci, localised on ten different chromosomes. These loci each accounted for between 2. 4 and 28. 6 % of the total genotypic variation for first flowering and response to vernalisation. However, identification of consistent QTL was found to be dependant upon growing environments. We compared the locations of QTL with the physical positions of predicted flowering time genes located on the sequenced genome of B. rapa. Some QTL associated with flowering time on A02, A03, A07, and C06 may represent homologues of known flowering time genes in Arabidopsis; VERNALISATION INSENSITIVE 3, APETALA1, CAULIFLOWER, FLOWERING LOCUS C, FLOWERING LOCUS T, CURLY LEAF, SHORT VEGETATIVE PHASE, GA3 OXIDASE, and LEAFY. Identification of the chromosomal location and effect of the genes influencing flowering time may hasten the development of canola varieties having an optimal time for flowering in target environments such as for low rainfall areas, via marker-assisted selection.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online: 7 September 2012.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Centre for Integrative Legume Research Publications
School of Agriculture and Food Sciences
Official 2013 Collection
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Citation counts: TR Web of Science Citation Count  Cited 27 times in Thomson Reuters Web of Science Article | Citations
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