Chaperone-usher fimbriae of Escherichia coli

Wurpel, Daniel J., Beatson, Scott A., Totsika, Makrina, Petty, Nicola K. and Schembri, Mark A. (2013) Chaperone-usher fimbriae of Escherichia coli. PLoS One, 85 1: e52835.1-e52835.11. doi:10.1371/journal.pone.0052835

Author Wurpel, Daniel J.
Beatson, Scott A.
Totsika, Makrina
Petty, Nicola K.
Schembri, Mark A.
Title Chaperone-usher fimbriae of Escherichia coli
Formatted title
Chaperone-usher fimbriae of Escherichia coli
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2013-01-30
Year available 2013
Sub-type Article (original research)
DOI 10.1371/journal.pone.0052835
Open Access Status DOI
Volume 85
Issue 1
Start page e52835.1
End page e52835.11
Total pages 11
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Collection year 2014
Language eng
Abstract Chaperone-usher (CU) fimbriae are adhesive surface organelles common to many Gram-negative bacteria. Escherichia coli genomes contain a large variety of characterised and putative CU fimbrial operons, however, the classification and annotation of individual loci remains problematic. Here we describe a classification model based on usher phylogeny and genomic locus position to categorise the CU fimbrial types of E. coli. Using the BLASTp algorithm, an iterative usher protein search was performed to identify CU fimbrial operons from 35 E. coli (and one Escherichia fergusonnii) genomes representing different pathogenic and phylogenic lineages, as well as 132 Escherichia spp. plasmids. A total of 458 CU fimbrial operons were identified, which represent 38 distinct fimbrial types based on genomic locus position and usher phylogeny. The majority of fimbrial operon types occupied a specific locus position on the E. coli chromosome; exceptions were associated with mobile genetic elements. A group of core-associated E. coli CU fimbriae were defined and include the Type 1, Yad, Yeh, Yfc, Mat, F9 and Ybg fimbriae. These genes were present as intact or disrupted operons at the same genetic locus in almost all genomes examined. Evaluation of the distribution and prevalence of CU fimbrial types among different pathogenic and phylogenic groups provides an overview of group specific fimbrial profiles and insight into the ancestry and evolution of CU fimbriae in E. coli.
Keyword Chaperone-usher (CU) fimbriae
Escherichia coli
Classification model
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Chemistry and Molecular Biosciences
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Citation counts: TR Web of Science Citation Count  Cited 35 times in Thomson Reuters Web of Science Article | Citations
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Created: Fri, 08 Feb 2013, 13:37:32 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences