Growth of epizootic hemorrhagic disease, akabane, and ephemeral fever viruses in Aedes albopictus cells maintained at various temperatures

Hoffman, D. and St George, T. D. (1985) Growth of epizootic hemorrhagic disease, akabane, and ephemeral fever viruses in Aedes albopictus cells maintained at various temperatures. Australian Journal of Biological Science, 38 2: 183-188.

Author Hoffman, D.
St George, T. D.
Title Growth of epizootic hemorrhagic disease, akabane, and ephemeral fever viruses in Aedes albopictus cells maintained at various temperatures
Journal name Australian Journal of Biological Science   Check publisher's open access policy
ISSN 0004-9417
1031-3613
Publication date 1985-01-01
Sub-type Article (original research)
Volume 38
Issue 2
Start page 183
End page 188
Total pages 6
Place of publication Collingwood, VIC, Australia
Publisher CSIRO Publishing
Language eng
Formatted abstract
The growth curves of one epizootic hemorrhagic disease (EHD) virus serotype (Reoviridae), two Akabane virus strains (Bunyaviridae) and three bovine ephemeral fever (BEF) group viruses (Rhabdoviridae) were determined in Aedes albopictus cells maintained at 15, 20, 28 and 33 degrees C. Ae albopictus cells supported the growth of all the viruses although not necessarily at all temperatures. Because none of the viruses exhibited cytopathic effect in Ae albopictus cells, growth was assayed in baby hamster kidney 21 (BHK21) cells maintained at 37 degrees C. The temperature at which the Ae albopictus cells were maintained had a marked effect on the growth and yield for each virus studied. EHD virus was heat-stable and grew after 4 days at 28 and 33 degrees C, and after 8 days at 20 degrees C. No growth was recorded up to 12 days at 15 degrees C. The two Akabane viruses were heat-sensitive and exhibited different growth patterns. One strain (B8935) showed no growth at 15 degrees C and only minimal growth at 20, 28 and 33 degrees C. The other strain (CSIRO 16) showed growth after 1-2 days at all temperatures with higher titres reached at 15 and 20 degrees C than at 28 and 33 degrees C. The BEF group viruses grew to approximately the same titres at all temperatures. At the higher temperatures (28 and 33 degrees C) most of BEF group viruses had disappeared within 9 days. In contrast at the lower temperatures (15 and 20 degrees C), there was still virus present 18 days after inoculation. BEF virus grew at 28°C after being maintained in Ae albopictus cells held at 4°C for 14 and 42 days.
Keyword Orbivirus
Arbovirus
Q-Index Code C1
Additional Notes Journal continues: Reproduction, Fertility and Development ISSN 1031-3613

Document type: Journal Article
Sub-type: Article (original research)
Collection: Toby Dix St George Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 3 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 0 times in Scopus Article
Google Scholar Search Google Scholar
Created: Thu, 07 Feb 2013, 23:57:19 EST by Ms Ramona Hooyer on behalf of Scholarly Communication and Digitisation Service