The isolation and preliminary characterization of a rhabdovirus in Australia related to bovine ephemeral fever virus

Cybinski, D. H. and Zakrzewski, H. (1983) The isolation and preliminary characterization of a rhabdovirus in Australia related to bovine ephemeral fever virus. Veterinary Microbiology, 8 3: 221-235. doi:10.1016/0378-1135(83)90075-5


Author Cybinski, D. H.
Zakrzewski, H.
Title The isolation and preliminary characterization of a rhabdovirus in Australia related to bovine ephemeral fever virus
Journal name Veterinary Microbiology   Check publisher's open access policy
ISSN 0378-1135
1873-2542
Publication date 1983-01-01
Sub-type Article (original research)
DOI 10.1016/0378-1135(83)90075-5
Volume 8
Issue 3
Start page 221
End page 235
Total pages 15
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Abstract CSIRO 368 virus was isolated from blood collected in the Northern Territory from a healthy cow and electron microscope studies showed that the isolate had rhabdovirus morphology. Fluorescent antibody studies and complement fixation tests related the virus to bovine ephemeral fever (BEF) virus. Neutralization tests in both suckling mice and Vero cells showed that the virus was not BEF virus. Antibodies to CSIRO 368 virus were found in cattle sera from northern and eastern Australia and Papua New Guinea. Antibodies were found in 16 out of 45 buffalo, some of which also had antibodies to BEF virus. In contrast, none of the 419 deer tested had antibodies to CSIRO 368 virus, although 142 of the same deer had antibodies to BEF virus. No antibodies to CSIRO 368 virus was detected in 16 goats, 54 horses, 10 pigs, 31 sheep, 25 kangaroos, of 14 human beings. Both CSIRO 368 and BEF virus were found to be sensitive to ether and chloroform, but were not affected by the DNA inhibitor 5-bromo-2'-deoxyuridine, showing that they probably had the same type of nucleic acid - namely RNA. CSIRO 368 was also shown to grow to higher titres in BHK21 cells than in Vero cells. Temperature sensitivity studies at -20, 4 and 37°C showed that the presence of foetal calf serum increased the survival time markedly at -20°C, but only slightly at 4 and 37°C. The virus survived the longest at -20°C in the presence of foetal calf serum.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Toby Dix St George Publications
 
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