Structure-function analysis of the GmRIC1 signal peptide and CLE domain required for nodulation control in soybean

Reid, Dugald E., Li, Dongxue, Ferguson, Brett J. and Gresshoff, Peter M. (2013) Structure-function analysis of the GmRIC1 signal peptide and CLE domain required for nodulation control in soybean. Journal of Experimental Botany, 64 6: 1575-1585. doi:10.1093/jxb/ert008


Author Reid, Dugald E.
Li, Dongxue
Ferguson, Brett J.
Gresshoff, Peter M.
Title Structure-function analysis of the GmRIC1 signal peptide and CLE domain required for nodulation control in soybean
Formatted title
Structure–function analysis of the GmRIC1 signal peptide and CLE domain required for nodulation control in soybean
Journal name Journal of Experimental Botany   Check publisher's open access policy
ISSN 0022-0957
1460-2431
Publication date 2013-04
Year available 2013
Sub-type Article (original research)
DOI 10.1093/jxb/ert008
Volume 64
Issue 6
Start page 1575
End page 1585
Total pages 11
Place of publication Oxford, United Kingdom
Publisher Oxford University Press
Collection year 2014
Language eng
Abstract Legumes control the nitrogen-fixing root nodule symbiosis in response to external and internal stimuli, such as nitrate, and via systemic autoregulation of nodulation (AON). Overexpression of the CLV3/ESR-related (CLE) pre-propeptideencoding genes GmNIC1 (nitrate-induced and acting locally) and GmRIC1 (Bradyrhizobium-induced and acting systemically) suppresses soybean nodulation dependent on the activity of the nodulation autoregulation receptor kinase (GmNARK). This nodule inhibition response was used to assess the relative importance of key structural components within and around the CLE domain sequences of these genes. Using a site-directed mutagenesis approach, mutants were produced at each amino acid within the CLE domain (RLAPEGPDPHHN) of GmRIC1. This approach identified the Arg1, Ala3, Pro4, Gly6, Pro7, Asp8, His11, and Asn12 residues as critical to GmRIC1 nodulation suppression activity (NSA). In contrast, none of the mutations in conserved residues outside of the CLE domain showed compromised NSA. Chimeric genes derived from combinations of GmRIC1 and GmNIC1 domains were used to determine the role of each pre-propeptide domain in NSA differences that exist between the two peptides. It was found that the transit peptide and CLE peptide regions of GmRIC1 significantly enhanced activity of GmNIC1. In contrast, the comparable GmNIC1 domains reduced the NSA of GmRIC1. Identification of these critical residues and domains provides a better understanding of how these hormone-like peptides function in plant development and regulation.
Keyword Autoregulation of nodulation
CLE peptides
Legumes
Nodulation
Soybean
Symbiosis
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes First published online: 5 February 2013.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Centre for Integrative Legume Research Publications
School of Agriculture and Food Sciences
Official 2014 Collection
 
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Created: Mon, 04 Feb 2013, 16:21:41 EST by Dr Brett Ferguson on behalf of Centre for Integrative Legume Research