Stac3 is required for myotube formation and myogenic differentiation in vertebrate skeletal muscle

Bower, Neil I., de la Serrana, Daniel Garcia, Cole, Nicholas J., Hollway, Georgina E., Lee, Hung-Tai, Assinder, Stephen and Johnston, Ian A. (2012) Stac3 is required for myotube formation and myogenic differentiation in vertebrate skeletal muscle. Journal of Biological Chemistry, 287 52: 43936-43949. doi:10.1074/jbc.M112.361311

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads
UQ290019_OA.pdf Full text (open access) application/pdf 6.18MB 0

Author Bower, Neil I.
de la Serrana, Daniel Garcia
Cole, Nicholas J.
Hollway, Georgina E.
Lee, Hung-Tai
Assinder, Stephen
Johnston, Ian A.
Title Stac3 is required for myotube formation and myogenic differentiation in vertebrate skeletal muscle
Journal name Journal of Biological Chemistry   Check publisher's open access policy
ISSN 0021-9258
Publication date 2012-12
Year available 2012
Sub-type Article (original research)
DOI 10.1074/jbc.M112.361311
Open Access Status File (Publisher version)
Volume 287
Issue 52
Start page 43936
End page 43949
Total pages 14
Place of publication Bethesda, MD, United States
Publisher American Society for Biochemistry and Molecular Biology
Collection year 2013
Language eng
Formatted abstract
Stac3 was identified as a nutritionally regulated gene from an Atlantic salmon subtractive hybridization library with highest expression in skeletal muscle. Salmon Stac3 mRNAwas highly correlated with myogenin and myoD1a expression during differentiation of a salmon primary myogenic culture and was regulated by amino acid availability. In zebrafish embryos, stac3 was initially expressed in myotomal adaxial cells and in fast muscle fibers post-segmentation. Morpholino knockdown resulted in defects in myofibrillar protein assembly, particularly in slow muscle fibers, and decreased levels of the hedgehog receptor patched. The function of Stac3 was further characterized in vitro using the mammalian C2C12 myogenic cell line. Stac3 mRNA expression increased during the differentiation of the C2C12 myogenic cell line. Knockdownof Stac3 byRNAiinhibitedmyotubeformation,andmicroarray analysis revealed that transcripts involved in cell cycle, focal adhesion, cytoskeleton, and the pro-myogenic factors Igfbp-5 and Igf2 were down-regulated. RNAi-treated cells had suppressed Akt signaling and exogenous insulin-like growth factor (Igf) 2 was unable to rescue the phenotype, however, Igf/Akt signaling was not blocked. Overexpression of Stac3, which results in increased levels of Igfbp-5 mRNA, did not lead to increased differentiation. In synchronized cells, Stac3 mRNA was most abundant during the G1 phase of the cell cycle. RNAi-treated cells were smaller, had higher proliferation rates and a decreased proportion of cells in G1 phase when compared with controls, suggesting a role in the G1 phase checkpoint. These results identify Stac3 as a new gene required for myogenic differentiation and myofibrillar protein assembly in vertebrates.
Keyword Stac3
Skeletal muscle
Myofibrillar protein
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
Institute for Molecular Bioscience - Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 16 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 15 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 27 Jan 2013, 00:14:02 EST by System User on behalf of Institute for Molecular Bioscience