Porphyra (Bangiophyceae) transcriptomes provide insights into red algal development and metabolism

Chan, Cheong Xin, Blouin, Nicolas A., Zhuang, Yunyun, Zaeuner, Simone, Prochnik, Simon E., Lindquist, Erika, Lin, Senjie, Benning, Christoph, Lohr, Martin, Yarish, Charles, Gantt, Elisabeth, Grossman, Arthur R., Lu, Shan, Mueller, Kirsten, Stiller, John W., Brawley, Susan H. and Bhattacharya, Debashish (2012) Porphyra (Bangiophyceae) transcriptomes provide insights into red algal development and metabolism. Journal of Phycology, 48 6: 1328-1342. doi:10.1111/j.1529-8817.2012.01229.x


Author Chan, Cheong Xin
Blouin, Nicolas A.
Zhuang, Yunyun
Zaeuner, Simone
Prochnik, Simon E.
Lindquist, Erika
Lin, Senjie
Benning, Christoph
Lohr, Martin
Yarish, Charles
Gantt, Elisabeth
Grossman, Arthur R.
Lu, Shan
Mueller, Kirsten
Stiller, John W.
Brawley, Susan H.
Bhattacharya, Debashish
Title Porphyra (Bangiophyceae) transcriptomes provide insights into red algal development and metabolism
Formatted title
Porphyra (Bangiophyceae) transcriptomes provide insights into red algal development and metabolism
Journal name Journal of Phycology   Check publisher's open access policy
ISSN 0022-3646
1529-8817
Publication date 2012-12
Sub-type Article (original research)
DOI 10.1111/j.1529-8817.2012.01229.x
Volume 48
Issue 6
Start page 1328
End page 1342
Total pages 15
Place of publication Hoboken, NJ, United States
Publisher Wiley-Blackwell
Collection year 2013
Language eng
Formatted abstract
The red seaweed Porphyra (Bangiophyceae) and related Bangiales have global economic importance. Here, we report the analysis of a comprehensive transcriptome comprising ca. 4.7 million expressed sequence tag (EST) reads from P. umbilicalis (L.) J. Agardh and P. purpurea (Roth) C. Agardh (ca. 980 Mbp of data generated using 454 FLX pyrosequencing). These ESTs were isolated from the haploid gametophyte (blades from both species) and diploid conchocelis stage (from P. purpurea). In a bioinformatic analysis, only 20% of the contigs were found to encode proteins of known biological function. Comparative analysis of predicted protein functions in mesophilic (including Porphyra) and extremophilic red algae suggest that the former has more putative functions related to signaling, membrane transport processes, and establishment of protein complexes. These enhanced functions may reflect general mesophilic adaptations. A near-complete repertoire of genes encoding histones and ribosomal proteins was identified, with some differentially regulated between the blade and conchocelis stage in P. purpurea. This finding may reflect specific regulatory processes associated with these distinct phases of the life history. Fatty acid desaturation patterns, in combination with gene expression profiles, demonstrate differences from seed plants with respect to the transport of fatty acid/lipid among subcellular compartments and the molecular machinery of lipid assembly. We also recovered a near-complete gene repertoire for enzymes involved in the formation of sterols and carotenoids, including candidate genes for the biosynthesis of lutein. Our findings provide key insights into the evolution, development, and biology of Porphyra, an important lineage of red algae.
Keyword Bangiales
Cellulose synthase
Extremophile
Fatty acid synthesis
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
Institute for Molecular Bioscience - Publications
 
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