Analysis of stomach bacterial communities in Australian feral horses

St-Pierre, Benoit, de la Fuente, Gabriel, O'Neill, Sean, Wright, Andre-Denis G. and Al Jassim, Rafat (2013) Analysis of stomach bacterial communities in Australian feral horses. Molecular Biology Reports, 40 1: 369-376. doi:10.1007/s11033-012-2070-5


Author St-Pierre, Benoit
de la Fuente, Gabriel
O'Neill, Sean
Wright, Andre-Denis G.
Al Jassim, Rafat
Title Analysis of stomach bacterial communities in Australian feral horses
Journal name Molecular Biology Reports   Check publisher's open access policy
ISSN 0301-4851
1573-4978
Publication date 2013-01
Year available 2012
Sub-type Article (original research)
DOI 10.1007/s11033-012-2070-5
Volume 40
Issue 1
Start page 369
End page 376
Total pages 8
Place of publication Dordrecht, Netherlands
Publisher Springer
Collection year 2013
Language eng
Abstract We investigated the community structure of bacteria that populate the stomach of the Brumby, a breed of feral horses from the Australian outback. Using a 16S rRNA gene clone library, we identified 155 clones that were assigned to 26 OTUs based on a 99.0% sequence identity cutoff. Two OTUs represented 73.5% of clones, while 18 OTUs were each assigned only a single clone. Four major bacterial types were identified in the Brumby stomach: Lactobacillaceae, Streptococcaceae, Veillonellaceae and Pasteurellaceae. The first three groups, which represented 98.1% of the Brumby stomach library clones, belonged to the bacterial phylum Firmicutes. We found that 49.7% of clones were related to bacterial species previously identified in the equine hindgut, and that 44.5% of clones were related to symbiotic bacterial species identified in the mouth or throat of either horses or other mammals. Our results indicated that the composition of mutualistic bacterial communities of feral horses was consistent with other studies on domestic horses. In addition to bacterial sequences, we also identified four plastid 16S rRNA gene sequences, which may help in further characterizing the type of vegetation consumed by Brumby horses in their natural environment.
Keyword Feral horse
16S rRNA
Mutualistic bacteria
Stomach
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online: 13 October 2012.

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2013 Collection
 
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