Antiangiogenesis and antioxidant activity of ethanol extracts of Pithecellobium jiringa

Muslim, Nahdzatul Syima, Nassar, Zeyad D., Aisha, Abdalrahim F. A., Shafaei, Armaghan, Idris, Norshirin, Majid, Amin Malik Shah Abdul and Ismail, Zhari (2012) Antiangiogenesis and antioxidant activity of ethanol extracts of Pithecellobium jiringa. Bmc Complementary and Alternative Medicine, 12 210.1-210.10. doi:10.1186/1472-6882-12-210


Author Muslim, Nahdzatul Syima
Nassar, Zeyad D.
Aisha, Abdalrahim F. A.
Shafaei, Armaghan
Idris, Norshirin
Majid, Amin Malik Shah Abdul
Ismail, Zhari
Title Antiangiogenesis and antioxidant activity of ethanol extracts of Pithecellobium jiringa
Journal name Bmc Complementary and Alternative Medicine   Check publisher's open access policy
ISSN 1472-6882
Publication date 2012-11
Sub-type Article (original research)
DOI 10.1186/1472-6882-12-210
Open Access Status DOI
Volume 12
Start page 210.1
End page 210.10
Total pages 10
Place of publication London, United Kingdom
Publisher BioMed Central
Collection year 2013
Language eng
Formatted abstract
Background: Angiogenesis plays a critical role in embryonic development and various physiological processes. However, excessive angiogenesis is associated with several pathological conditions including cancer. Pithecellobium jiringa (Jack) Prain is a traditional medicinal plant from the family Leguminosae. It is native to the Southeast Asia, where it has been used traditionally for treatment of various ailments such as hypertension and diabetes. The present work is aimed to study antioxidant and antiangiogenesis activities of P. jiringa ethanol extracts.

Methods: P. jiringa fruit rinds were extracted with ethanol and 50% ethanol. The antioxidant property was analysed using, 1,1-diphenyl-2-picryl-hydrazyl free radical scavenging assay. Phytochemical analysis was performed using thin layer chromatography and colorimetric methods. Then, cell growth inhibition was studied against a panel of human cell lines by MTT test. In vitro inhibition of angiogenesis was studied by the following assays: isolated rat aortic rings cell viability, colony formation, endothelial cell migration, endothelial tube formation on matrigel, and expression of vascular endothelial growth factor by endothelial cells. In vivo antiangiogenesis effect was studied by utilising fertilised chick embryos assay. The results were statistically analysed by analysis of variance.

Results: Ethanolic and 50% hydro-ethanolic extracts showed relatively high concentration of total phenolics associated with potent antioxidant activity. The rat aortic rings study conducted showed potent inhibition of the microvessels outgrowth with IC50s 5.27 ± 0.81 μg/ml (ethanolic) and 4.45 ± 0.63 μg/ml (50% hydro-ethanolic). Both extracts arrested the growth of human endothelial cells via down-regulation of VEGF expression, leading to inhibition of other angiogenesis cascades including migration of endothelial cells, and formation of capillary network on matrigel matrix. The extracts also inhibited the neovascularisation of chick embryo chorioallantoic membrane.

Conclusions: P. jiringa extracts inhibit angiogenesis by blocking the VEGF expression thus inhibiting endothelial cells proliferation, migration and differentiation most likely due to presence of the antioxidant phenolics.
Keyword Pithecellobium jiringa
Antiangiogenesis
Antioxidant
Phytochemical analysis
Proliferation
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article number 210.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Pharmacy Publications
 
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