Effect of different cellulase and pectinase enzyme treatments on protoplast isolation and viability in lilium ledebeourii bioss

Chamani, Esmaeil, Tahami, Seyyed Karim, Zare, Nasser, Asghari-Zakaria, Rasool, Mohebodini, Mehdi and Joyce, Daryl (2012) Effect of different cellulase and pectinase enzyme treatments on protoplast isolation and viability in lilium ledebeourii bioss. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 40 2: 123-128.

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Author Chamani, Esmaeil
Tahami, Seyyed Karim
Zare, Nasser
Asghari-Zakaria, Rasool
Mohebodini, Mehdi
Joyce, Daryl
Title Effect of different cellulase and pectinase enzyme treatments on protoplast isolation and viability in lilium ledebeourii bioss
Journal name Notulae Botanicae Horti Agrobotanici Cluj-Napoca   Check publisher's open access policy
ISSN 0255-965X
1842-4309
Publication date 2012
Sub-type Article (original research)
Open Access Status File (Publisher version)
Volume 40
Issue 2
Start page 123
End page 128
Total pages 6
Place of publication Cluj-Napoca, Romania
Publisher Academic Pres, Universitatea de Stiinte Agricole si Medicina Veterinara Cluj-Napoca
Collection year 2013
Language eng
Abstract For overcoming interspecific incompatibility, protoplast combination method is a proper procedure for making a new plant with desired traits. For this purpose, protoplast preparation is a first and important step. Hence, experiments were conducted to evaluate various combinations of cellulose, pectinase and their treatment times on protoplast production and protoplast viability in Lilium ledebeourii Bioss. The results of experiment revealed that the protoplast yield was significantly affected by different treatment levels. Cellulase at 4% gave the highest numbers of protoplasts at 3.71×10 5 protoplast/g FW. Pectinase at 1% gave the highest numbers of protoplast. For treatment times, the highest yield of protoplast was with leaf explants treated for 24 h. Analysis of variance indicated that concentration, time and three-way interaction of cellulase, pectinase and time were significant at p<0.01. Cellulase at 4% and pectinase at 0.2% for 24 h gave the highest viability. Interactions of cellulase × pectinase, cellulase × time, pectinase × time and cellulase × pectinase × treatment time were significant at P≤0.05 for protoplast number. The highest and lowest protoplast numbers were produced in media containing 4% cellulase and 1% pectinase for 24 h (6.65×105 protoplast/g FW) and 1% cellulase and 0.2% pectinase for 12 h, respectively. It's concluded that, the best treatment for isolation of Lilium protoplast was 4% cellulase and 1% pectinase for 24 h.
Keyword Cellulase
Lilium ledebeourii Bioss.
Pectinase
Protoplast isolation
Viability
Growth
Callus
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2013 Collection
 
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