Challenges in mass spectrometry-based quantification of bioactive peptides: A case study exploring the neuropeptide Y family

Xi, Li, Jin, Yaping, Parker, Edward A., Josh, Peter, Jones, Alun, Wijffels, Gene and Colgrave, Michelle L. (2012) Challenges in mass spectrometry-based quantification of bioactive peptides: A case study exploring the neuropeptide Y family. Biopolymers, 98 4: 357-366. doi:10.1002/bip.22109


Author Xi, Li
Jin, Yaping
Parker, Edward A.
Josh, Peter
Jones, Alun
Wijffels, Gene
Colgrave, Michelle L.
Title Challenges in mass spectrometry-based quantification of bioactive peptides: A case study exploring the neuropeptide Y family
Journal name Biopolymers   Check publisher's open access policy
ISSN 0006-3525
1097-0282
Publication date 2012-06-22
Sub-type Article (original research)
DOI 10.1002/bip.22109
Volume 98
Issue 4
Start page 357
End page 366
Total pages 10
Place of publication Hoboken, NJ, United States
Publisher John Wiley & Sons
Collection year 2013
Language eng
Formatted abstract
The study of biologically active peptides is critical to the understanding of physiological pathways, especially those involved in the development of disease. Historically, themeasurement of biologically active endogenous peptides has been undertaken by radioimmunoassay, a highly sensitive and robust technique that permits the detectionof physiological concentrations in different biofluid and tissue extracts. Over recent years, a range of mass spectrometric approaches have been applied to peptide quantification with limited degrees of success. Neuropeptide Y (NPY), peptide  YY (PYY), and pancreatic polypeptide (PP) belong to the NPY family exhibiting   regulatory effects on appetite and feeding behavior. The physiological significance of these peptides depends on their molecular forms and in vivo concentrations systemically and at local sites within tissues. In this report, we describe an approach for quantification of individual peptides within mixtures using high-performance liquid chromatography electrospray ionization tandem mass spectrometry analysis of the  NPY family peptides. Aspects of quantification including sample preparation, the use of matrix-matched calibration curves, and internal standards will be discussed. This method for the simultaneous determination of NPY, PYY, and PP was accurate and  reproducible but lacks the sensitivity required for measurement of their endogenous concentration in plasma. The advantages of mass spectrometric quantification will be discussed alongside the current obstacles and challenges.
Keyword Neuropeptide Y
peptide YY
pancreatic polypeptide
multiple reaction monitoring
quantification
Absolute Quantification
Pancreatic-Polypeptide
In-Vivo
Electrospray-Ionization
Obese Women
Food-Intake
Plasma
Proteins
Proteomics
Serum
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
Institute for Molecular Bioscience - Publications
 
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