SNX5 is essential for efficient macropinocytosis and antigen processing in primary macrophages

Lim, Jet Phey, Teasdale, Rohan D. and Gleeson, Paul A. (2012) SNX5 is essential for efficient macropinocytosis and antigen processing in primary macrophages. Biology Open, 1 9: 904-914. doi:10.1242/bio.20122204

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Author Lim, Jet Phey
Teasdale, Rohan D.
Gleeson, Paul A.
Title SNX5 is essential for efficient macropinocytosis and antigen processing in primary macrophages
Journal name Biology Open   Check publisher's open access policy
ISSN 2046-6390
Publication date 2012-09
Sub-type Article (original research)
DOI 10.1242/bio.20122204
Open Access Status DOI
Volume 1
Issue 9
Start page 904
End page 914
Total pages 11
Place of publication Cambridge, United Kingdom
Publisher The Company of Biologists
Collection year 2013
Language eng
Formatted abstract
Macropinocytosis mediates the bulk endocytosis of solute molecules, nutrients and antigens. As this endocytic pathway is considered important in functions associated with immune responses, the molecular mechanisms regulating this pathway in immune cells is of particular significance. However, the regulators of macropinocytosis in primary cells remain poorly defined. Members of the sorting nexin (SNX) family have been implicated in macropinosome biogenesis in cultured cells and here we have analyzed the role of two SNX family members, SNX1 and its binding partner SNX5, in macropinocytosis of mouse primary macrophages. We show that endogenous SNX1 and SNX5 are localised to newly-formed macropinosomes in primary mouse macrophages and, moreover, demonstrate that SNX5 plays an essential role in macropinosome biogenesis. Depletion of SNX5 in bone marrow-derived macrophages dramatically decreased both the number and size of macropinosomes. Depletion of SNX5 also resulted in dramatic reduction in uptake and processing of soluble ovalbumin in macrophages, indicating that the majority of antigen uptake and delivery to late endosomes is via macropinocytosis. By contrast, the absence of SNX1 had no effect on endogenous SNX5 localisation and macropinosome biogenesis using macrophages from SNX1 knockout mice. Therefore, SNX5 can function independently of SNX1 and is a modulator of macropinocytosis that influences the uptake and processing of soluble antigen in primary mouse macrophages.
Keyword Macropinocytosis
Sorting nexins
Endocytosis
Antigen processing
SNX5
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
Institute for Molecular Bioscience - Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 4 times in Thomson Reuters Web of Science Article | Citations
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Created: Fri, 14 Dec 2012, 11:02:03 EST by Susan Allen on behalf of Institute for Molecular Bioscience