siRNA against the G gene of human metapneumovirus

Preston, Faith Maxine, Straub, Claire P., Ramirez, Ruben, Mahalingam, Suresh and Spann, Kirsten M. (2012) siRNA against the G gene of human metapneumovirus. Virology Journal, 9 105.1-105.5. doi:10.1186/1743-422X-9-105


Author Preston, Faith Maxine
Straub, Claire P.
Ramirez, Ruben
Mahalingam, Suresh
Spann, Kirsten M.
Title siRNA against the G gene of human metapneumovirus
Journal name Virology Journal   Check publisher's open access policy
ISSN 1743-422X
Publication date 2012-06
Sub-type Article (original research)
DOI 10.1186/1743-422X-9-105
Open Access Status DOI
Volume 9
Start page 105.1
End page 105.5
Total pages 5
Place of publication London, United Kingdom
Publisher BioMed Central
Collection year 2013
Language eng
Formatted abstract
Background: Human metapneumovirus (hMPV) is a significant viral respiratory pathogen of infants and children, the elderly and immunocompromised individuals. Disease associated with hMPV infection resembles that of human respiratory syncytial virus (RSV) and includes bronchiolitis and pneumonia. The glycosylated G attachment protein of hMPV is required for viral entry in vivo and has also been identified as an inhibitor of innate immune responses.
Findings: We designed and validated two siRNA molecules against the G gene using A549 cells and demonstrated consistent 88-92% knock-down for one siRNA molecule, which was used in subsequent experiments. Significant reduction of G mRNA in A549 cells infected with hMPV did not result in a reduction in viral growth, nor did it significantly increase the production of type I interferon (α/β) in response to infection. However, there was a moderate increase in IFN-β mRNA expression in response to infection in siG-transfected cells compared to untransfected and si-mismatch-transfected cells. Expression of G by recombinant adenovirus did not affect type I IFN expression.
Conclusion: G has been previously described as a type I interferon antagonist, although our findings suggest it may not be a significant antagonist.
Keyword siRNA
hMPV
Type I interferon
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article # 105. Published under Short report.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
Clinical Medical Virology Centre Publications
 
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Created: Mon, 10 Dec 2012, 10:17:45 EST by Lesley Arnicar on behalf of Clinical Medical Virology Centre