In planta localization and interactions of impatiens necrotic spot tospovirus proteins

Dietzgen, Ralf G., Martin, Kathleen M., Anderson, Gavin and Goodin, Michael M. (2012) In planta localization and interactions of impatiens necrotic spot tospovirus proteins. Journal of General Virology, 93 PART 11: 2490-2495. doi:10.1099/vir.0.042515-0


Author Dietzgen, Ralf G.
Martin, Kathleen M.
Anderson, Gavin
Goodin, Michael M.
Title In planta localization and interactions of impatiens necrotic spot tospovirus proteins
Journal name Journal of General Virology   Check publisher's open access policy
ISSN 0022-1317
1465-2099
Publication date 2012-11
Sub-type Article (original research)
DOI 10.1099/vir.0.042515-0
Volume 93
Issue PART 11
Start page 2490
End page 2495
Total pages 6
Place of publication Reading, Berkshire, United Kingdom
Publisher Society for General Microbiology
Collection year 2013
Language eng
Abstract Impatiens necrotic spot tospovirus (INSV) is a significant pathogen of ornamentals. The tripartite negative- and ambi-sense RNA genome encodes six proteins that are involved in cytoplasmic replication, movement, assembly, insect transmission and defence. To gain insight into the associations of these viral proteins, we determined their intracellular localization and interactions in living plant cells. Nucleotide sequences encoding the nucleoprotein N, non-structural proteins NSs and NSm, and glycoproteins Gn and Gc of a Kentucky isolate of INSV were amplified by RTPCR, cloned, sequenced and transiently expressed as fusions with autofluorescent proteins in leaf epidermal cells of Nicotiana benthamiana. All proteins accumulated at the cell periphery and co-localized with an endoplasmic reticulum marker. The Gc protein fusion also localized to the nucleus. N and NSm protein self-interactions and an NSm-N interaction were observed by using bimolecular fluorescence complementation. A tospovirus NSm homotypic interaction had not been reported previously.
Keyword Tospovirus
Impatiens necrotic spot tospovirus
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
Official 2013 Collection
 
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