Induction of potent CD8(+) T cell responses through the delivery of subunit protein vaccines to skin antigen-presenting cells using densely packed microprojection arrays

Ng, Hwee-Ing, Fernando, Germain J. P. and Kendall, Mark A. F. (2012) Induction of potent CD8(+) T cell responses through the delivery of subunit protein vaccines to skin antigen-presenting cells using densely packed microprojection arrays. Journal of Controlled Release, 162 3: 477-484. doi:10.1016/j.jconrel.2012.07.024


Author Ng, Hwee-Ing
Fernando, Germain J. P.
Kendall, Mark A. F.
Total Author Count Override 3
Title Induction of potent CD8(+) T cell responses through the delivery of subunit protein vaccines to skin antigen-presenting cells using densely packed microprojection arrays
Formatted title
Induction of potent CD8+ T cell responses through the delivery of subunit protein vaccines to skin antigen-presenting cells using densely packed microprojection arrays
Journal name Journal of Controlled Release   Check publisher's open access policy
ISSN 0168-3659
1873-4995
Publication date 2012-09
Sub-type Article (original research)
DOI 10.1016/j.jconrel.2012.07.024
Volume 162
Issue 3
Start page 477
End page 484
Total pages 8
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2013
Language eng
Formatted abstract
The generation of both antibody and CD8 + T cell responses against pathogens is considered important for many advanced vaccines for diseases including tuberculosis, HIV and malaria. However, most current vaccines are delivered into muscle by the needle and syringe method and induce protection via humoral (antibody) immune responses. In this paper, we test the hypothesis that delivering a model subunit protein antigen (ovalbumin) to the skin's abundant immune cell population using a densely packed microprojection array (Nanopatch) enhances CD8 + T cell responses. We found that the Nanopatch significantly enhanced the CD8 + T cell responses when compared to intramuscular delivery of both antigen-only and adjuvanted cases (Quil-A and CpG; separately). To our knowledge, this is the first published study demonstrating significantly improved CD8 + T cell responses achieved by delivering subunit vaccines to the skin's abundant immune cell population. Successfully replicating these findings in humans could significantly advance the reach of vaccines.
Keyword Needle-free vaccine
Dose sparing
Adjuvant
Microneedle
Transdermal
Ctl
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online: 27 July 2012.

 
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