Synthesis of DNA Oligonucleotides containing C5-Ethynylbenzenesulfonamide-modified nucleotides (EBNA) by polymerases towards the construction of base functionalized nucleic acids

Goubet, Astrid, Chardon, Antoine, Kumar, Pawan, Sharma, Pawan K. and Veedu, Rakesh N. (2013) Synthesis of DNA Oligonucleotides containing C5-Ethynylbenzenesulfonamide-modified nucleotides (EBNA) by polymerases towards the construction of base functionalized nucleic acids. Bioorganic & Medicinal Chemistry Letters, 23 3: 761-763. doi:10.1016/j.bmcl.2012.11.096


Author Goubet, Astrid
Chardon, Antoine
Kumar, Pawan
Sharma, Pawan K.
Veedu, Rakesh N.
Title Synthesis of DNA Oligonucleotides containing C5-Ethynylbenzenesulfonamide-modified nucleotides (EBNA) by polymerases towards the construction of base functionalized nucleic acids
Journal name Bioorganic & Medicinal Chemistry Letters   Check publisher's open access policy
ISSN 0960-894X
1464-3405
Publication date 2013-02
Year available 2012
Sub-type Article (original research)
DOI 10.1016/j.bmcl.2012.11.096
Volume 23
Issue 3
Start page 761
End page 763
Total pages 3
Place of publication Kidlington, Oxford, United Kingdom
Publisher Pergamon
Collection year 2013
Language eng
Abstract C5-Ethynylbenzenesulfonamide-modified nucleotide (EBNA) was investigated as substrate of various DNA polymerases. The experiments revealed that KOD, Phusion and Klenow DNA polymerases successfully accepted EBNA-T nucleotide as a substrate and yielded the fully extended DNA. KOD DNA polymerase was found to be the most efficient enzyme to furnish EBNA-T containing DNA in good yields. Phusion DNA polymerase efficiently amplified the template containing EBNA-T nucleotides by PCR.
Keyword Modified
Nucleotides Enzymatic
Incorporation Functionalized
Nucleic
Acids Oligonucleotides
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online: 3 December 2012.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Chemistry and Molecular Biosciences
 
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Created: Fri, 07 Dec 2012, 09:25:44 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences