The synthesis and degradation of collagenase-degradable poly(2-hydroxyethyl methacrylate)-based hydrogels and sponges for potential applications as scaffolds in tissue engineering

Paterson, Stefan M., Shadforth, Audra M. A., Brown, David H., Madden, Peter W., Chirila, Traian V. and Baker, Murray V. (2012) The synthesis and degradation of collagenase-degradable poly(2-hydroxyethyl methacrylate)-based hydrogels and sponges for potential applications as scaffolds in tissue engineering. Materials Science and Engineering C: Materials for Biological Applications, 32 8: 2536-2544. doi:10.1016/j.msec.2012.07.037


Author Paterson, Stefan M.
Shadforth, Audra M. A.
Brown, David H.
Madden, Peter W.
Chirila, Traian V.
Baker, Murray V.
Title The synthesis and degradation of collagenase-degradable poly(2-hydroxyethyl methacrylate)-based hydrogels and sponges for potential applications as scaffolds in tissue engineering
Journal name Materials Science and Engineering C: Materials for Biological Applications   Check publisher's open access policy
ISSN 0928-4931
1873-0191
Publication date 2012-12
Sub-type Article (original research)
DOI 10.1016/j.msec.2012.07.037
Volume 32
Issue 8
Start page 2536
End page 2544
Total pages 9
Place of publication Lausanne, Switzerland
Publisher Elsevier
Collection year 2013
Language eng
Abstract A collagenase-cleavable peptide-based crosslinking agent was synthesized and was incorporated into PHEMA sponges, and P[HEMA-co-MeO-PEGMA] gels and sponges [HEMA 2-hydroxyethyl methacrylate, PHEMA = poly(2-hydroxyethyl methacrylate), MeO-PEGMA = poly(ethylene glycol) monomethyl ether methacrylate]. PHEMA and P[HEMA-co-MeO-PEGMA] sponges had polymer droplet morphologies where the dimensions of the morphological features were three to five times larger compared to sponges that were crosslinked with tetraethylene glycol dimethacrylate (TEGDMA), while the P[HEMA-co-MeO-PEGMA] gels had similar morphologies regardless of the crosslinking agent. The differences in the dimensions of the morphologies of the sponges were attributed to differences in hydrophilicities of the crosslinking agent. When incubated in a collagenase solution, PHEMA sponges did not degrade, but P[HEMA-co-MeO-PEGMA] gels took 28 days to degrade and the P[HEMA-co-MeO-PEGMA] sponges took 101 days to degrade to 8% dry weight remaining. A cytotoxicity assay showed that the hydrogels do not elicit any cytotoxic response in vitro.
Keyword PHEMA
Functionalized peptides
Enzymatic biodegradation
Collagenase
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online: 28 July 2012.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Medicine Publications
Australian Institute for Bioengineering and Nanotechnology Publications
 
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