Intrasample variation and the effect of storage delay on faecal metabolite concentrations in the southern hairy-nosed wombat (Lasiorhinus latifrons)

Descovich, Kristin A., Lisle, Allan T., Johnston, Stephen, Keeley, Tamara and Phillips, Clive J. C. (2012) Intrasample variation and the effect of storage delay on faecal metabolite concentrations in the southern hairy-nosed wombat (Lasiorhinus latifrons). Australian Mammalogy, 34 2: 217-222. doi:10.1071/AM11047


Author Descovich, Kristin A.
Lisle, Allan T.
Johnston, Stephen
Keeley, Tamara
Phillips, Clive J. C.
Title Intrasample variation and the effect of storage delay on faecal metabolite concentrations in the southern hairy-nosed wombat (Lasiorhinus latifrons)
Formatted title
Intrasample variation and the effect of storage delay on faecal metabolite concentrations in the southern hairy-nosed wombat (Lasiorhinus latifrons)
Journal name Australian Mammalogy   Check publisher's open access policy
ISSN 0310-0049
1836-7402
Publication date 2012-05
Sub-type Article (original research)
DOI 10.1071/AM11047
Open Access Status
Volume 34
Issue 2
Start page 217
End page 222
Total pages 6
Place of publication Collingwood, VIC Australia
Publisher C S I R O Publishing
Collection year 2013
Language eng
Formatted abstract
Faecal hormone metabolite analysis offers a non-invasive alternative to blood sampling that is particularly relevant to wild animal populations. It has been used for reproductive and adrenal hormone monitoring in captive wombats, but no information exists on its accuracy when sample storage is delayed or on whether variation exists within samples. This study aimed to quantify the effect of storage delay and intrasample variation on faecal metabolite concentrations of testosterone, progesterone and corticosterone in southern hairy-nosed wombats. For all metabolites, intrasample variance was 15% or less of total variance. Delays in storage significantly changed metabolite concentrations, with progesterone showing a progressive decrease over time (072h: t24≤2.14, P≤0.05), testosterone showing a significant increase after 6-h delay (06h: t21≤2.83, P≤0.01), and corticosterone showing an initial increase (06h: t48≤3.60, P≤0.001), followed by a reduction to original concentrations by 72h (072h: t48≤0.91, P≤0.38). Although changes caused by storage delays may not obscure large hormonal fluctuations, this study outlines the importance of immediate storage and consistent sampling to allow the most accurate results to be obtained. Journal compilation
Keyword Corticosterone
Hormone analysis
Progesterone
Testosterone
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2013 Collection
School of Veterinary Science Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 3 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 3 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 02 Dec 2012, 00:30:56 EST by System User on behalf of School of Agriculture and Food Sciences