Interplay between manganese and iron in pneumococcal pathogenesis: role of the orphan response regulator RitR

Ong, Cheryl-lynn Y., Potter, Adam J., Trappetti, Claudia, Walker, Mark J., Jennings, Michael P., Paton, James C. and McEwan, Alastair G. (2013) Interplay between manganese and iron in pneumococcal pathogenesis: role of the orphan response regulator RitR. Infection and Immunity, 81 2: 421-429. doi:10.1128/IAI.00805-12

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Author Ong, Cheryl-lynn Y.
Potter, Adam J.
Trappetti, Claudia
Walker, Mark J.
Jennings, Michael P.
Paton, James C.
McEwan, Alastair G.
Title Interplay between manganese and iron in pneumococcal pathogenesis: role of the orphan response regulator RitR
Journal name Infection and Immunity   Check publisher's open access policy
ISSN 1098-5522
1070-6313
Publication date 2013-02
Year available 2012
Sub-type Article (original research)
DOI 10.1128/IAI.00805-12
Open Access Status File (Publisher version)
Volume 81
Issue 2
Start page 421
End page 429
Total pages 9
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2013
Language eng
Formatted abstract
Streptococcus pneumoniae (the pneumococcus) is a major human pathogen that is carried asymptomatically in the nasopharynx by up to 70% of the human population. Translocation of the bacteria into internal sites can cause a range of diseases such as pneumonia, otitis media, meningitis and bacteraemia. This transition from nasopharynx to growth at systemic sites means that penumococcus needs to adjust to a variety of environmental conditions including transition metal ion availabilty. Although it is an important nutrient, iron potentiates oxidative stress and it is established that in S. pneumoniae, expression of iron transport systems and proteins that protect against oxidative stress are regulated by an orphan response regulator, RitR. In this study, we investigated the effect of iron and manganese ion availbility on the growth of a ritR mutant. Deletion of ritR led to impaired growth of bacteria in high iron medium but this phenotype could be suppressed with the addition of manganese. Measurement of metal ion accumulation indicated that manganese prevents iron accumulation. Furthermore, the addition of manganese also led to a reduction in the amount of hydrogen peroxide produced by bacterial cells. Studies of virulence in a murine model of infection indicated that RitR was not essential for pneumococcal survival and suggested that derepression of iron uptake systems may enhance survival of pneumococcus in some niches.
Keyword Pneumococcus
Response regulator
Iron
Manganese
Oxidative stress
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online ahead of print: 26 November 2012.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Chemistry and Molecular Biosciences
 
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Created: Fri, 30 Nov 2012, 13:12:14 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences