Development of chromosome-arm-specific microsatellite markers in Triticum aestivum (Poaceae) using Ngs technology

Nie, Xiaojun, Li, Bianli, Wang, Le, Liu, Peixun, Biradar, Siddanagouda S., Li, Tao, Dolezel, Jaroslav, Edwards, David, Luo, Mingcheng and Song Weining (2012) Development of chromosome-arm-specific microsatellite markers in Triticum aestivum (Poaceae) using Ngs technology. American Journal of Botany, 99 9: E369-E371. doi:10.3732/ajb.1200077


Author Nie, Xiaojun
Li, Bianli
Wang, Le
Liu, Peixun
Biradar, Siddanagouda S.
Li, Tao
Dolezel, Jaroslav
Edwards, David
Luo, Mingcheng
Song Weining
Title Development of chromosome-arm-specific microsatellite markers in Triticum aestivum (Poaceae) using Ngs technology
Formatted title
Development of chromosome-arm-specific microsatellite markers in Triticum aestivum (Poaceae) using Ngs technology
Journal name American Journal of Botany   Check publisher's open access policy
ISSN 0002-9122
1537-2197
Publication date 2012-09-01
Sub-type Article (original research)
DOI 10.3732/ajb.1200077
Volume 99
Issue 9
Start page E369
End page E371
Total pages 3
Place of publication St. Louis, MO, United States
Publisher Botanical Society of America
Collection year 2013
Language eng
Formatted abstract
• Premise of the study:
The aim of this study was to assess the feasibility of developing chromosome-arm-specific microsatellite markers in wheat on a large scale based on chromosome survey sequences obtained with next-generation sequencing (NGS) technology.

• Methods and Results:
The Illumina Hi Seq2000 sequencing platform was used to sequence DNA of isolated wheat chromosome- arm 7DL. The data were assembled and microsatellite loci were identified computationally. In total, 16 315 microsatellites were identified from 161 061 assembled contigs. Thirty-three markers were randomly selected for validation across 20 diverse wheat cultivars. Two nulli-tetrasomic stocks were also screened to validate the specificity of the newly developed markers.

• Conclusions:

This is the first study on identification of chromosome-arm-specific microsatellite markers using NGS technology. These new chromosome-arm-specific markers will facilitate saturation of the 7DL genetic map, and their availability will support genetic mapping and positional cloning in wheat.
Keyword Chromosome-arm-specific DNA
Flow-sorted chromosomes
Next-generation sequencing
Poaceae
Triticum aestivum
Wheat
Ssr markers
Wheat
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2013 Collection
 
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