The X-ray crystal structure of a pseudoazurin from Sinorhizobium meliloti

Laming, Elise M., McGrath, Aaron P., Guss, J. Mitchell, Kappler, Ulrike and Maher, Megan J. (2012) The X-ray crystal structure of a pseudoazurin from Sinorhizobium meliloti. Journal of Inorganic Biochemistry, 115 148-154. doi:10.1016/j.jinorgbio.2012.04.005


Author Laming, Elise M.
McGrath, Aaron P.
Guss, J. Mitchell
Kappler, Ulrike
Maher, Megan J.
Title The X-ray crystal structure of a pseudoazurin from Sinorhizobium meliloti
Journal name Journal of Inorganic Biochemistry   Check publisher's open access policy
ISSN 0162-0134
Publication date 2012-10
Sub-type Article (original research)
DOI 10.1016/j.jinorgbio.2012.04.005
Volume 115
Start page 148
End page 154
Total pages 7
Collection year 2013
Language eng
Abstract The X-ray crystal structure of oxidised pseudoazurin from the denitrifying plant symbiotic bacterium Sinorhizobium meliloti (SmPAz2) has been solved to a resolution of 2.0 Å. The pseudoazurin from Sinorhizobium sp. is unusual as it forms an operon with a sulfite dehydrogenase enzyme, rather than a Cu nitrite reductase. Examination of the structure reveals that the geometric parameters of the Type I Cu site in SmPAz2 correlate with observed features in the electronic spectrum of the protein. Comparison of the structure of SmPAz2 with those of pseudoazurins from five other bacterial species shows that the surface of SmPAz2 bears a conserved hydrophobic patch encircled by positively-charged residues, which may serve as a recognition site for its redox partners.
Keyword Blue copper protein
Pseudoazurin
Sulfite oxidation
X-ray crystallography
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online: 1 May 2012.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Chemistry and Molecular Biosciences
 
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Created: Fri, 23 Nov 2012, 10:56:36 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences