A genome-wide screen to identify transcription factors expressed in pelvic ganglia of the lower urinary tract

Weise, Carrie B., Ireland, Sara, Fleming, Nicole L., Yu, Jing, Valerius, M. Todd, Kylie Georgas, Chiu, Han Sheng, Brennan, Jane, Armstrong, Jane, Little, Melissa H., McMahon, Andrew P. and Southard-Smith, E. Michelle (2012) A genome-wide screen to identify transcription factors expressed in pelvic ganglia of the lower urinary tract. Frontiers in Neuroscience, SEP: . doi:10.3389/fnins.2012.00130

Author Weise, Carrie B.
Ireland, Sara
Fleming, Nicole L.
Yu, Jing
Valerius, M. Todd
Kylie Georgas
Chiu, Han Sheng
Brennan, Jane
Armstrong, Jane
Little, Melissa H.
McMahon, Andrew P.
Southard-Smith, E. Michelle
Title A genome-wide screen to identify transcription factors expressed in pelvic ganglia of the lower urinary tract
Journal name Frontiers in Neuroscience   Check publisher's open access policy
ISSN 1662-4548
Publication date 2012-09-12
Sub-type Article (original research)
DOI 10.3389/fnins.2012.00130
Open Access Status DOI
Issue SEP
Total pages 15
Place of publication Lausanne, Switzerland
Publisher Frontiers Research Foundation
Collection year 2013
Language eng
Formatted abstract
Relative positions of neurons within mature murine pelvic ganglia based on expression of neurotransmitters have been described. However the spatial organization of developing innervation in the murine urogenital tract (UGT) and the gene networks that regulate specification and maturation of neurons within the pelvic ganglia of the lower urinary tract (LUT) are unknown.We used whole-mount immunohistochemistry and histochemical stains to localize neural elements in 15.5 days post coitus (dpc) fetal mice. To identify potential regulatory factors expressed in pelvic ganglia, we surveyed expression patterns for known or probable transcription factors (TF) annotated in the mouse genome by screening a wholemount
in situ hybridization library of fetal UGTs. Of the 155 genes detected in pelvic ganglia,
88 encodeTFs based on the presence of predicted DNA-binding domains. Neural crest (NC)-derived progenitors within the LUT were labeled by Sox10, a well-known regulator of NC development. Genes identified were categorized based on patterns of restricted expression in pelvic ganglia, pelvic ganglia and urethral epithelium, or pelvic ganglia and urethral mesenchyme. Gene expression patterns and the distribution of Sox10C, Phox2bC, HuC, and PGP9.5C cells within developing ganglia suggest previously unrecognized regional segregation of Sox10C progenitors and differentiating neurons in early development of pelvic ganglia. Reverse transcription-PCR of pelvic ganglia RNA from fetal and post-natal stages demonstrated that multiple TFs maintain post-natal expression, although Pax3 is
extinguished before weaning. Our analysis identifies multiple potential regulatory genes includingTFs that may participate in segregation of discrete lineages within pelvic ganglia.  The genes identified here are attractive candidate disease genes that may now be further investigated for their roles in malformation syndromes or in LUT dysfunction.
Keyword Pelvic Ganglia
Autonomic nervous system
Transcription Factor
Lower Urinary tract
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
Institute for Molecular Bioscience - Publications
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Citation counts: TR Web of Science Citation Count  Cited 7 times in Thomson Reuters Web of Science Article | Citations
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Created: Fri, 16 Nov 2012, 14:05:58 EST by Susan Allen on behalf of Institute for Molecular Bioscience