Screening of 71 P. multocida proteins for protective efficacy in a fowl cholera infection model and characterization of the protective antigen PlpE

Hatfaludi, Tamas, Al-Hasani, Keith, Gong, Lan, Boyce, John D., Ford, Mark, Wilkie, Ian W., Quinsey, Noelene, Dunstone, Michelle A., Hoke, David E. and Adler, Ben (2012) Screening of 71 P. multocida proteins for protective efficacy in a fowl cholera infection model and characterization of the protective antigen PlpE. Plos One, 7 7 Article No. e39973: . doi:10.1371/journal.pone.0039973


Author Hatfaludi, Tamas
Al-Hasani, Keith
Gong, Lan
Boyce, John D.
Ford, Mark
Wilkie, Ian W.
Quinsey, Noelene
Dunstone, Michelle A.
Hoke, David E.
Adler, Ben
Title Screening of 71 P. multocida proteins for protective efficacy in a fowl cholera infection model and characterization of the protective antigen PlpE
Journal name Plos One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2012-07
Sub-type Article (original research)
DOI 10.1371/journal.pone.0039973
Open Access Status DOI
Volume 7
Issue 7 Article No. e39973
Total pages 11
Place of publication San Francisco, CA United States
Publisher Public Library of Science
Collection year 2013
Language eng
Formatted abstract
Background: There is a strong need for a recombinant subunit vaccine against fowl cholera. We used a reverse vaccinology approach to identify putative secreted or cell surface associated P. multocida proteins that may represent potential vaccine candidate antigens. Principal Findings: A high-throughput cloning and expression protocol was used to express and purify 71 recombinant proteins for vaccine trials. Of the 71 proteins tested, only one, PlpE in denatured insoluble form, protected chickens against fowl cholera challenge. PlpE also elicited comparable levels of protection in mice. PlpE was localized by immunofluorescence to the bacterial cell surface, consistent with its ability to elicit a protective immune response. To explore the role of PlpE during infection and immunity, a plpE mutant was generated. The plpE mutant strain retained full virulence for mice. Conclusion: These studies show that PlpE is a surface exposed protein and was the only protein of 71 tested that was able to elicit a protective immune response. However, PlpE is not an essential virulence factor. This is the first report of a denatured recombinant protein stimulating protection against fowl cholera.
Keyword Gram negative bacteria
Group B streptococcus
Haemolytica Serotype 1
Hemorrhagic Septicemia
Subcellular Localization
Vaccine Development
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Veterinary Science Publications
 
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