Prominence of an O75 clonal group (clonal complex 14) among non-st131 fluoroquinolone-resistant Escherichia coli causing extraintestinal infections in humans and dogs in Australia

Platell, Joanne L., Trott, Darren J., Johnson, James R., Heisig, Peter, Heisig, Anke, Clabots, Connie R., Johnston, Brian and Cobbold, Rowland N. (2012) Prominence of an O75 clonal group (clonal complex 14) among non-st131 fluoroquinolone-resistant Escherichia coli causing extraintestinal infections in humans and dogs in Australia. Antimicrobial Agents and Chemotherapy, 56 7: 3898-3904. doi:10.1128/AAC.06120-11

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Author Platell, Joanne L.
Trott, Darren J.
Johnson, James R.
Heisig, Peter
Heisig, Anke
Clabots, Connie R.
Johnston, Brian
Cobbold, Rowland N.
Title Prominence of an O75 clonal group (clonal complex 14) among non-st131 fluoroquinolone-resistant Escherichia coli causing extraintestinal infections in humans and dogs in Australia
Journal name Antimicrobial Agents and Chemotherapy   Check publisher's open access policy
ISSN 0066-4804
1098-6596
Publication date 2012-07
Sub-type Article (original research)
DOI 10.1128/AAC.06120-11
Open Access Status File (Publisher version)
Volume 56
Issue 7
Start page 3898
End page 3904
Total pages 7
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2013
Language eng
Formatted abstract
Fluoroquinolone (FQ)-resistant extraintestinal pathogenic Escherichia coli (FQr ExPEC) strains from phylogenetic group B2 are undergoing epidemic spread. Isolates belonging to phylogenetic group B2 are generally more virulent than other E. coli isolates; therefore, resistance to FQs among group B2 isolates is concerning. Although clonal expansion of sequence type 131 (ST131) is a major factor, the contribution of additional clonal groups has not been quantified. Group B2 FQr ExPEC isolates from humans (n = 250) and dogs (n = 12) in Australia were screened for ST131, a recently recognized and rapidly emerging multidrug-resistant and virulent clonal group that is important in both human and companion animal medicine. Non-ST131 isolates underwent virulence genotyping, PCR-based O typing, partial multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and FQ resistance mechanism analysis. Of 49 non-ST131 isolates (45 human, 4 canine), 49% (24 human, 2 canine) represented O-type O75 and exhibited conserved virulence genotypes (F10 papA allele, iha, fimH, sat, vat, fyuA, iutA, kpsMII, usp, ompT, malX, K1/K5 capsule) and MLST allele profiles corresponding with clonal complex CC14. Two clusters, each containing canine and human isolates, were identified by PFGE (differentiated by K1 and K5 capsules). Australian FQr O75 isolates exhibited commonality with an historical FQ-susceptible O75 urosepsis isolate (also CC14). The isolation from humans and dogs of highly similar FQr derivatives of the classic O75:K1/K5 (CC14) ExPEC lineage suggests recent acquisition of FQ resistance and potential cross-host-species transfer. This lineage should be targeted with ST131 in future epidemiological investigations of FQr ExPEC.
Keyword Urinary-tract-infections
Field gel-electrophoresis
Sequence type St131
Companion animals
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Veterinary Science Publications
 
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