Oligomeric amyloid-beta peptide affects the expression of genes involved in steroid and lipid metabolism in primary neurons

Malik, Bilal, Fernandes, Cathy, Killick, Richard, Wroe, Richard, Usardi, Alessia, Williamson, Ritchie, Kellie, Stuart, Anderton, Brian H. and Reynolds, C. Hugh (2012) Oligomeric amyloid-beta peptide affects the expression of genes involved in steroid and lipid metabolism in primary neurons. Neurochemistry International, 61 3: 321-333. doi:10.1016/j.neuint.2012.05.006


Author Malik, Bilal
Fernandes, Cathy
Killick, Richard
Wroe, Richard
Usardi, Alessia
Williamson, Ritchie
Kellie, Stuart
Anderton, Brian H.
Reynolds, C. Hugh
Title Oligomeric amyloid-beta peptide affects the expression of genes involved in steroid and lipid metabolism in primary neurons
Formatted title
Oligomeric amyloid-β peptide affects the expression of genes involved in steroid and lipid metabolism in primary neurons
Journal name Neurochemistry International   Check publisher's open access policy
ISSN 0197-0186
1872-9754
Publication date 2012-08
Sub-type Article (original research)
DOI 10.1016/j.neuint.2012.05.006
Volume 61
Issue 3
Start page 321
End page 333
Total pages 13
Place of publication London, United Kingdom
Publisher Elsevier
Collection year 2013
Language eng
Formatted abstract
Amyloid-β peptide (Aβ) is the principal component of plaques in the brains of patients with Alzheimer's disease (AD), and the most toxic form of Aβ may be as soluble oligomers. We report here the results of a microarray study of gene expression profiles in primary mouse cortical neurons in response to oligomeric Aβ 1-42. A major and unexpected finding was the down-regulation of genes involved in the biosynthesis of cholesterol and other steroids and lipids (such as Fdft1, Fdps, Idi1, Ldr, Mvd, Mvk, Nsdhl, Sc4mol), the expression of which was verified by quantitative real-time RT-PCR (qPCR). The ATP-binding cassette gene Abca1, which has a major role in cholesterol transport in brain and other tissues and has been genetically linked to AD, was notably up-regulated. The possible involvement of cholesterol and other lipids in Aβ synthesis and action in Alzheimer's disease has been studied and debated extensively but remains unresolved. These new data suggest that Aβ may influence steroid and lipid metabolism in neurons via multiple gene-expression changes.
Keyword Amyloid beta oligomers
ADDLs
Microarray
Gene expression
Cholesterol
Steroid metabolism
Alzheimers-Disease
Cholesterol-Metabolism
Neuroblastoma-Cells
Synaptic Plasticity
Oxidative Stress
Transgenic Mice
Protein Set
Mouse Model
In-Vivo
Abca1
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Chemistry and Molecular Biosciences
Institute for Molecular Bioscience - Publications
 
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