Methanogen Colonisation Does Not Significantly Alter Acetogen Diversity in Lambs Isolated 17 h After Birth and Raised Aseptically

Gagen, Emma J., Mosoni, Pascale, Denman, Stuart E., Al Jassim, Rafat, McSweeney, Christopher S. and Forano, Evelyne (2012) Methanogen Colonisation Does Not Significantly Alter Acetogen Diversity in Lambs Isolated 17 h After Birth and Raised Aseptically. Microbial Ecology, 64 3: 628-640. doi:10.1007/s00248-012-0024-z


Author Gagen, Emma J.
Mosoni, Pascale
Denman, Stuart E.
Al Jassim, Rafat
McSweeney, Christopher S.
Forano, Evelyne
Title Methanogen Colonisation Does Not Significantly Alter Acetogen Diversity in Lambs Isolated 17 h After Birth and Raised Aseptically
Journal name Microbial Ecology   Check publisher's open access policy
ISSN 0095-3628
1432-184X
Publication date 2012-10
Sub-type Article (original research)
DOI 10.1007/s00248-012-0024-z
Volume 64
Issue 3
Start page 628
End page 640
Total pages 13
Place of publication Secaucus, NJ, United States
Publisher Springer
Collection year 2013
Language eng
Formatted abstract
Reductive acetogenesis is not competitive with methanogenesis in adult ruminants, whereas acetogenic bacteria are the dominant hydrogenotrophs in the early rumen microbiota. The ecology of hydrogenotrophs in the developing rumen was investigated using young lambs, raised in sterile isolators, and conventional adult sheep. Two lambs were born naturally, left with their dams for 17 h and then placed into a sterile isolator and reared aseptically. They were inoculated with cellulolytic bacteria and later with Methanobrevibacter sp. 87.7 to investigate the effect of methanogen establishment on the rumen acetogen population since they lacked cultivable representatives of methanogens. Putative acetogens were investigated by acetyl-CoA synthase and formyltetrahydrofolate synthetase gene analysis and methanogens by methyl coenzyme reductase A gene analysis. Unexpectedly, a low abundant but diverse population of methanogens (predominantly Methanobrevibacter spp.) was identified in isolated lambs pre-inoculation with Mbb. sp 87.7, which was similar to the community structure in conventional sheep. In contrast, potential acetogen diversity in isolated lambs and conventional sheep was different. Potential acetogens affiliated between the Lachnospiraceae and Clostridiaceae in conventional sheep and with the Blautia genus and the Lachnospiraceae in isolated lambs. The establishment of Mbb. sp. 87.7 (1,000-fold increase in methanogens) did not substantially affect acetogen diversity.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2013 Collection
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 15 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 16 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Thu, 15 Nov 2012, 12:02:42 EST by System User on behalf of School of Animal Studies