Comparative pathogenomics reveals horizontally acquired novel virulence genes in fungi infecting cereal hosts

Gardiner, Donald M., McDonald, Megan C., Covarelli, Lorenzo, Solomon, Peter S., Rusu, Anca G., Marshall, Mhairi, Kazan, Kemal, Chakraborty, Sukumar, McDonald, Bruce A. and Manners, John M. (2012) Comparative pathogenomics reveals horizontally acquired novel virulence genes in fungi infecting cereal hosts. Plos Pathogens, 8 9: e1002952.1-e1002952.22. doi:10.1371/journal.ppat.1002952


Author Gardiner, Donald M.
McDonald, Megan C.
Covarelli, Lorenzo
Solomon, Peter S.
Rusu, Anca G.
Marshall, Mhairi
Kazan, Kemal
Chakraborty, Sukumar
McDonald, Bruce A.
Manners, John M.
Title Comparative pathogenomics reveals horizontally acquired novel virulence genes in fungi infecting cereal hosts
Journal name Plos Pathogens   Check publisher's open access policy
ISSN 1553-7374
Publication date 2012-09
Sub-type Article (original research)
DOI 10.1371/journal.ppat.1002952
Open Access Status DOI
Volume 8
Issue 9
Start page e1002952.1
End page e1002952.22
Total pages 22
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Collection year 2013
Language eng
Formatted abstract
Comparative analyses of pathogen genomes provide new insights into how pathogens have evolved common and divergent virulence strategies to invade related plant species. Fusarium crown and root rots are important diseases of wheat and barley world-wide. In Australia, these diseases are primarily caused by the fungal pathogen Fusarium pseudograminearum. Comparative genomic analyses showed that the F. pseudograminearum genome encodes proteins that are present in other fungal pathogens of cereals but absent in non-cereal pathogens. In some cases, these cereal pathogen specific genes were also found in bacteria associated with plants. Phylogenetic analysis of selected F. pseudograminearum genes supported the hypothesis of horizontal gene transfer into diverse cereal pathogens. Two horizontally acquired genes with no previously known role in fungal pathogenesis were studied functionally via gene knockout methods and shown to significantly affect virulence of F. pseudograminearum on the cereal hosts wheat and barley. Our results indicate using comparative genomics to identify genes specific to pathogens of related hosts reveals novel virulence genes and illustrates the importance of horizontal gene transfer in the evolution of plant infecting fungal pathogens.
Keyword Wheat crown rot
Fusarium-graminearum
Stagonospora-nodorum
Mycotoxin deoxynivalenol
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
UQ Diamantina Institute Publications
 
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