Toxicokinetic and toxicodynamic model for diazinon toxicity-mechanistic explanation of differences in the sensitivity of Daphnia magna and Gammarus pulex

Kretschmann, Andreas, Ashauer, Roman, Hollender, Juliane and Escher, Beate I. (2012) Toxicokinetic and toxicodynamic model for diazinon toxicity-mechanistic explanation of differences in the sensitivity of Daphnia magna and Gammarus pulex. Environmental Toxicology and Chemistry, 31 9: 2014-2022. doi:10.1002/etc.1905


Author Kretschmann, Andreas
Ashauer, Roman
Hollender, Juliane
Escher, Beate I.
Title Toxicokinetic and toxicodynamic model for diazinon toxicity-mechanistic explanation of differences in the sensitivity of Daphnia magna and Gammarus pulex
Journal name Environmental Toxicology and Chemistry   Check publisher's open access policy
ISSN 0730-7268
1552-8618
Publication date 2012-09
Sub-type Article (original research)
DOI 10.1002/etc.1905
Volume 31
Issue 9
Start page 2014
End page 2022
Total pages 9
Place of publication Hoboken, NJ, United States
Publisher John Wiley & Sons
Collection year 2013
Language eng
Formatted abstract
A mechanistic toxicokinetic and toxicodynamic model for acute toxic effects (immobilization, mortality) of the organothiophosphate insecticide diazinon in Daphnia magna is presented. The model was parameterized using measured external and internal (whole-body) concentrations of diazinon, its toxic metabolite diazoxon, and the inactive metabolite 2-isopropyl-6-methyl-4-pyrimidinol, plus acetylcholinesterase (AChE) activity measured during exposure to diazinon in vivo. The toxicokinetic and toxicodynamic model provides a coherent picture from exposure to the resulting toxic effect on an organism level through internally formed metabolites and the effect on a molecular scale. A very fast reaction of diazoxon with AChE (pseudo first-order inhibition rate constant ki = 3.3 h−1) compared with a slow formation of diazoxon (activation rate constant kact = 0.014 h−1) was responsible for the high sensitivity of D. magna toward diazinon. Recovery of AChE activity from inhibition was slow and rate-determining (99% recovery within 16 d), compared with a fast elimination of diazinon (99% elimination within 17 h). The obtained model parameters were compared with toxicokinetic and toxicodynamic parameters of Gammarus pulex exposed to diazinon from previous work. This comparison revealed that G. pulex is less sensitive because of a six times faster detoxification of diazinon and diazoxon and an approximately 400 times lower rate for damage accrual. These differences overcompensate the two times faster activation of diazinon to diazoxon in G. pulex compared to D. magna. The present study substantiates theoretical considerations that mechanistically based effect models are helpful to explain sensitivity differences among different aquatic invertebrates.
Keyword Diazinon
Daphnia magna
Gammarus pulex
Toxicokinetic and toxicodynamic model
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
National Research Centre for Environmental Toxicology Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
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