Ontological Differences in First Compared to Third Trimester Human Fetal Placental Chorionic Stem Cells

Jones, Gemma N., Moschidou, Dafni, Puga-Iglesias, Tamara-Isabel, Kuleszewicz, Katarzyna, Vanleene, Maximilien, Shefelbine, Sandra J., Bou-Gharios, George, Fisk, Nicholas M., David, Anna L., De Coppi, Paolo and Guillot, Pascale V. (2012) Ontological Differences in First Compared to Third Trimester Human Fetal Placental Chorionic Stem Cells. PLoS One, 7 9 Article No. e43395: . doi:10.1371/journal.pone.0043395

Author Jones, Gemma N.
Moschidou, Dafni
Puga-Iglesias, Tamara-Isabel
Kuleszewicz, Katarzyna
Vanleene, Maximilien
Shefelbine, Sandra J.
Bou-Gharios, George
Fisk, Nicholas M.
David, Anna L.
De Coppi, Paolo
Guillot, Pascale V.
Total Author Count Override 11
Title Ontological Differences in First Compared to Third Trimester Human Fetal Placental Chorionic Stem Cells
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2012-09
Sub-type Article (original research)
DOI 10.1371/journal.pone.0043395
Open Access Status DOI
Volume 7
Issue 9 Article No. e43395
Total pages 15
Place of publication San Francisco, CA United States
Publisher Public Library of Science
Collection year 2013
Language eng
Formatted abstract
Human mesenchymal stromal/stem cells (MSC) isolated from fetal tissues hold promise for use in tissue engineering applications and cell-based therapies, but their collection is restricted ethically and technically. In contrast, the placenta is a potential source of readily-obtainable stem cells throughout pregnancy. In fetal tissues, early gestational stem cells are known to have advantageous characteristics over neonatal and adult stem cells. Accordingly, we investigated whether early fetal placental chorionic stem cells (e-CSC) were physiologically superior to their late gestation fetal chorionic counterparts (l-CSC). We showed that e-CSC shared a common phenotype with l-CSC, differentiating down the osteogenic, adipogenic and neurogenic pathways, and containing a subset of cells endogenously expressing NANOG, SOX2, c-MYC, and KLF4, as well as an array of genes expressed in pluripotent stem cells and primordial germ cells, including CD24, NANOG, SSEA4, SSEA3, TRA-1-60, TRA-1-81, STELLA, FRAGILIS, NANOS3, DAZL and SSEA1. However, we showed that e-CSC have characteristics of an earlier state of stemness compared to l-CSC, such as smaller size, faster kinetics, uniquely expressing OCT4A variant 1 and showing higher levels of expression of NANOG, SOX2, c-MYC and KLF4 than l-CSC. Furthermore e-CSC, but not l-CSC, formed embryoid bodies containing cells from the three germ layer lineages. Finally, we showed that e-CSC demonstrate higher tissue repair in vivo; when transplanted in the osteogenesis imperfecta mice, e-CSC, but not l-CSC increased bone quality and plasticity; and when applied to a skin wound, e-CSC, but not l-CSC, accelerated healing compared to controls. Our results provide insight into the ontogeny of the stemness phenotype during fetal development and suggest that the more primitive characteristics of early compared to late gestation fetal chorionic stem cells may be translationally advantageous.
Keyword Primordial germ cells
Mesenchymal Stromal Cells
Adult Bone Marrow
Progenitor Cells
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2013 Collection
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Citation counts: TR Web of Science Citation Count  Cited 21 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 28 times in Scopus Article | Citations
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