Longitudinal investigation of natural killer cells and cytokines in chronic fatigue syndrome/myalgic encephalomyelitis

Brenu, Ekua W., van Driel, Mieke L., Staines, Donald R., Ashton, Kevin J., Hardcastle, Sharni L., Keane, James, Tajouri, Lotti, Peterson, Daniel, Ramos, Sandra B. and Marshall-Gradisnik, Sonya M. (2012) Longitudinal investigation of natural killer cells and cytokines in chronic fatigue syndrome/myalgic encephalomyelitis. Journal of Translational Medicine, 10 1: 88.1-88.11. doi:10.1186/1479-5876-10-88


Author Brenu, Ekua W.
van Driel, Mieke L.
Staines, Donald R.
Ashton, Kevin J.
Hardcastle, Sharni L.
Keane, James
Tajouri, Lotti
Peterson, Daniel
Ramos, Sandra B.
Marshall-Gradisnik, Sonya M.
Title Longitudinal investigation of natural killer cells and cytokines in chronic fatigue syndrome/myalgic encephalomyelitis
Journal name Journal of Translational Medicine   Check publisher's open access policy
ISSN 1479-5876
Publication date 2012-05
Sub-type Article (original research)
DOI 10.1186/1479-5876-10-88
Open Access Status DOI
Volume 10
Issue 1
Start page 88.1
End page 88.11
Total pages 11
Place of publication London, U.K.
Publisher BioMed Central
Collection year 2013
Language eng
Formatted abstract
Background: Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME) is an etiologically unexplained disorder characterised by irregularities in various aspects of the immunological function. Presently, it is unknown whether these immunological changes remain consistent over time. This study investigates Natural Killer (NK) cell cytotoxic activity, NK cell subsets (CD56brightCD16- and CD56dimCD16+) and cytokines, over the course of a12 month period in patients with CFS/ME.
Methods: The participants in the study comprised 65 (47.2+/-11.5 years) CFS/ME participants and 21 (45.2 +/-9.3 years) non-fatigued controls. Flow cytometry protocols were used to assess NK subsets and NK cytotoxic activity at various time points that included baseline (T1), 6 (T2) and 12 months (T3). Cytokine secretions were measured following mitogenic stimulation of peripheral blood mononuclear cells.
Results: NK cytotoxic activity was significantly decreased in the CFS/ME patients at T1, T2 and T3 compared to the non-fatigued group. Additionally, in comparison to the non-fatigued controls, the CFS/ME group had significantly lower numbers of CD56brightCD16- NK cells at both T1 and T2. Interestingly, following mitogenic stimulation, cytokine secretion revealed significant increases in IL-10, IFN-gamma and TNF-alpha at T1 in the CFS/ME group. A significant decrease was observed at T2 in the CFS/ME group for IL-10 and IL-17A while at T3, IL-2 was increased in the CFS/ME group in comparison to the non- fatigued controls. Overall cytotoxic activity was significantly decreased at T3 compared to T1 and T2. CD56brightCD16- NK cells were much lower at T2 compared to the T1 and T3. IL-10 and IL-17A secretion was elevated at T2 in comparison to the T1 and T3.
Conclusion: These results confirm decreases in immune function in CFS/ME patients, suggesting an increased susceptibility to viral and other infections. Furthermore NK cytotoxic activity may be a suitable biomarker for diagnosing CFS/ME as it was consistently decreased during the course of the 12 months study.
Keyword Chronic fatigue syndrome
Cytokines
Cytotoxic activity
Macrophage-derived chemokine
Immunological abnormalities
T-Cells
Multiple-sclerosis
Lymphocyte subsets
Adaptive immunity
Major depression
Nk cells
Receptors
Innate
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article 88

Document type: Journal Article
Sub-type: Article (original research)
Collections: Discipline of General Practice Publications
Official 2013 Collection
School of Medicine Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 30 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 40 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Thu, 15 Nov 2012, 11:31:46 EST by System User on behalf of School of Medicine