Prostaglandin E-2 affects osteoblast biology in a dose-dependent manner: an in vitro study

Ramirez-Yanez, G. O. and Symons, A. L. (2012) Prostaglandin E-2 affects osteoblast biology in a dose-dependent manner: an in vitro study. Archives of Oral Biology, 57 9: 1274-1281. doi:10.1016/j.archoralbio.2012.03.003

Author Ramirez-Yanez, G. O.
Symons, A. L.
Title Prostaglandin E-2 affects osteoblast biology in a dose-dependent manner: an in vitro study
Journal name Archives of Oral Biology   Check publisher's open access policy
ISSN 0003-9969
Publication date 2012-09
Sub-type Article (original research)
DOI 10.1016/j.archoralbio.2012.03.003
Volume 57
Issue 9
Start page 1274
End page 1281
Total pages 8
Place of publication Kidlington, Oxford, United Kingdom
Publisher Pergamon
Collection year 2013
Language eng
Formatted abstract
Objective: This study aimed to determine in vitro how exogenous PGE 2 affects the expression of genes in cultured osteoblasts by relative quantitation PCR.

Design: Cultured osteoblasts were exposed to 10 -3 M, 10 -5 M or 10 -7 M PGE 2 over 5, 10, 15 and 20 days. Results: RANKL expression was higher after 5 days of exposure (p < 0.05), but thereafter reduced in those treated with the two lower doses of PGE 2 (p < 0.01). RANKL/OPG ratio reported in favour of OPG gene expression and alkaline phosphatase gene expression increased in osteoblasts exposed to the two lower doses of the eicosanoid after 15 days. Conversely, prostaglandin E synthase, a cytokine produced during PGE 2 synthesis, gene expression was significantly reduced at 15 and 20 days (p < 0.01 and 0.05 respectively). The results from this study add to the current knowledge of the mechanisms by which PGE 2 modulates the osteoblast biology in a dose-dependent manner.

Conclusions: It is proposed that PGE 2at a low dose switch osteoblast's biology in favour of bone apposition by: first, inducing a significantly higher OPG gene expression overwhelming RANKL gene expression; second, reducing PGEs synthesis; and third, increasing ALP gene expression. An opposite effect is expected when the concentration of the eicosanoid overpass certain levels.
Keyword Prostaglandin E-2
Bone biology
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Dentistry Publications
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