An improved experimental model for understanding the impact of sperm DNA fragmentation on human pregnancy following ICSI

Nunez-Calonge, Rocio, Caballero, Pedro, Lopez-Fernandez, Carmen, Andres Guijarro, J., Luis Fernandez, Jose, Johnston, Stephen and Gosalvez, Jaime (2012) An improved experimental model for understanding the impact of sperm DNA fragmentation on human pregnancy following ICSI. Reproductive Sciences, 19 11: 1163-1168.


Author Nunez-Calonge, Rocio
Caballero, Pedro
Lopez-Fernandez, Carmen
Andres Guijarro, J.
Luis Fernandez, Jose
Johnston, Stephen
Gosalvez, Jaime
Title An improved experimental model for understanding the impact of sperm DNA fragmentation on human pregnancy following ICSI
Journal name Reproductive Sciences   Check publisher's open access policy
ISSN 1933-7191
1933-7205
Publication date 2012-11
Sub-type Article (original research)
DOI 10.1177/1933719112459238
Volume 19
Issue 11
Start page 1163
End page 1168
Total pages 6
Place of publication Thousand Oaks, CA, United States
Publisher Sage
Collection year 2013
Language eng
Abstract Using donor oocytes of proven fertility, the effect of sperm DNA fragmentation (SDF) and motility on reproductive success was examined in 70 couples undergoing ICSI. Both SDF and sperm motility were assessed at the time of sperm injection and using the same sperm sample that was processed for ICSI. While there was no difference in the fertilization rate, cleavage rate, embryo quality, or sperm motility between pregnant and nonpregnant couples, the SDF of nonpregnant couples (SDF = 23.9%) was higher than that of pregnant couples (SDF = 17.0%; U Mann-Whitney 347; P =.002). Using a combination of the sensitivity and specificity measures from the production of ROC (receiver-operating characteristic) curves and the Youden index, we determined a threshold SDF value for our data set of 17% for predicting pregnancy (77.8% sensitivity and 71.1% specificity). Our results suggest that proven donor oocytes in combination with SDF assessment at the time of sperm injection represent a useful experimental model for reducing the confounding influences of sperm DNA repair by the oocyte and iatrogenic sperm damage.
Keyword Sperm DNA fragmentation
DNA repair
Oocyte
ICSI
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2013 Collection
 
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