Koi herpesvirus encodes and expresses a functional interleukin-10

Sunarto, Agus, Liongue, Clifford, McColl, Kenneth A., Adams, Mathew M., Bulach, Dieter, Crane, Mark St. J., Schat, Karel A., Slobedman, Barry, Barnes, Andrew C., Ward, Alister C. and Walker, Peter J. (2012) Koi herpesvirus encodes and expresses a functional interleukin-10. Journal of Virology, 86 21: 11512-11520. doi:10.1128/JVI.00957-12

Author Sunarto, Agus
Liongue, Clifford
McColl, Kenneth A.
Adams, Mathew M.
Bulach, Dieter
Crane, Mark St. J.
Schat, Karel A.
Slobedman, Barry
Barnes, Andrew C.
Ward, Alister C.
Walker, Peter J.
Title Koi herpesvirus encodes and expresses a functional interleukin-10
Journal name Journal of Virology   Check publisher's open access policy
ISSN 0022-538X
Publication date 2012-11
Year available 2012
Sub-type Article (original research)
DOI 10.1128/JVI.00957-12
Open Access Status DOI
Volume 86
Issue 21
Start page 11512
End page 11520
Total pages 9
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2013
Language eng
Abstract Koi herpesvirus (KHV) (species Cyprinid herpesvirus 3) ORF134 was shown to transcribe a spliced transcript encoding a 179-amino-acid (aa) interleukin-10 (IL-10) homolog (khvIL-10) in koi fin (KF-1) cells. Pairwise sequence alignment indicated that the expressed product shares 25% identity with carp IL-10, 22 to 24% identity with mammalian (including primate) IL-10s, and 19.1% identity with European eel herpesvirus IL-10 (ahvIL-10). In phylogenetic analyses, khvIL-10 fell in a divergent position from all host IL-10 sequences, indicating extensive structural divergence following capture from the host. In KHV-infected fish, khvIL-10 transcripts were observed to be highly expressed during the acute and reactivation phases but to be expressed at very low levels during low-temperature-induced persistence. Similarly, KHV early (helicase [Hel] and DNA polymerase [DNAP]) and late (intercapsomeric triplex protein [ITP] and major capsid protein [MCP]) genes were also expressed at high levels during the acute and reactivation phases, but only low-level expression of the ITP gene was detected during the persistent phase. Injection of khvIL-10 mRNA into zebrafish (Danio rerio) embryos increased the number of lysozyme-positive cells to a similar degree as zebrafish IL-10. Downregulation of the IL-10 receptor long chain (IL-10R1) using a specific morpholino abrogated the response to both khvIL-10 and zebrafish IL-10 transcripts, indicating that, despite the structural divergence, khvIL-10 functions via this receptor. This is the first report describing the characteristics of a functional viral IL-10 gene in the Alloherpesviridae.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Biological Sciences Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 16 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 21 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 14 Nov 2012, 16:10:07 EST by Gail Walter on behalf of School of Biological Sciences