Development of 11 microsatellite markers for Giraffa camelopardalis through 454 pyrosequencing, with primer options for an additional 458 microsatellites

Carter, Kerryn D., Seddon, Jennifer M., Carter, John K., Goldizen, Anne W. and Hereward, James P. (2012) Development of 11 microsatellite markers for Giraffa camelopardalis through 454 pyrosequencing, with primer options for an additional 458 microsatellites. Conservation Genetics Resources, 4 4: 943-945. doi:10.1007/s12686-012-9679-5

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Author Carter, Kerryn D.
Seddon, Jennifer M.
Carter, John K.
Goldizen, Anne W.
Hereward, James P.
Title Development of 11 microsatellite markers for Giraffa camelopardalis through 454 pyrosequencing, with primer options for an additional 458 microsatellites
Journal name Conservation Genetics Resources   Check publisher's open access policy
ISSN 1877-7252
1877-7260
Publication date 2012-05
Sub-type Article (original research)
DOI 10.1007/s12686-012-9679-5
Volume 4
Issue 4
Start page 943
End page 945
Total pages 3
Place of publication Dordrecht, Netherlands
Publisher Springer
Collection year 2013
Language eng
Abstract Many wild giraffe populations are declining across Africa, with two subspecies listed by the IUCN as Endangered in the past 4 years. We developed 11 microsatellite markers from Giraffa camelopardalis angolensis in Etosha National Park, Namibia using 454 sequencing. In 70 individuals, the loci showed 2-4 alleles per locus and expected heterozygosities of 0. 082-0. 711. There were no significant deviations from Hardy-Weinberg equilibrium for any of the loci. Null allele frequencies were low (<3 %) across all loci. We present primer options for an additional 458 microsatellites. This new set of microsatellite markers and primer options will benefit conservation, population and quantitative genetics studies of giraffe populations.
Keyword Giraffa camelopardalis
Giraffes
Microsatellite markers
454 pyrosequencing
Subspecies
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online: 26 May 2012.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Biological Sciences Publications
School of Veterinary Science Publications
 
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Created: Wed, 14 Nov 2012, 13:16:05 EST by Gail Walter on behalf of School of Biological Sciences