Antiviral protection and the importance of Wolbachia density and: tissue tropism in Drosophila simulans

Osborne, Sheree E., Iturbe-Ormaetxe, Inaki, Brownlie, Jeremy C., O'Neill, Scott L. and Johnson, Karyn N. (2012) Antiviral protection and the importance of Wolbachia density and: tissue tropism in Drosophila simulans. Applied and Environmental Microbiology, 78 19: 6922-6929.


Author Osborne, Sheree E.
Iturbe-Ormaetxe, Inaki
Brownlie, Jeremy C.
O'Neill, Scott L.
Johnson, Karyn N.
Title Antiviral protection and the importance of Wolbachia density and: tissue tropism in Drosophila simulans
Formatted title Antiviral protection and the importance of Wolbachia density and: tissue tropism in Drosophila simulans
Journal name Applied and Environmental Microbiology   Check publisher's open access policy
ISSN 0099-2240
1098-5336
Publication date 2012-10
Sub-type Article (original research)
DOI 10.1128/AEM.01727-12
Volume 78
Issue 19
Start page 6922
End page 6929
Total pages 8
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2013
Language eng
Formatted abstract Wolbachia, a maternally transmitted endosymbiont of insects, is increasingly being seen as an effective biological control agent that can interfere with transmission of pathogens, including dengue virus. However, the mechanism of antiviral protection is not well understood. The density and distribution of Wolbachia in host tissues have been implicated as contributing factors by previous studies with both mosquitoes and flies. Drosophila flies infected with five diverse strains of Wolbachia were screened for the ability to mediate antiviral protection. The three protective Wolbachia strains were more closely related and occurred at a higher density within whole flies than the two nonprotective Wolbachia strains. In this study, to further investigate the relationship between whole-fly Wolbachia density and the ability to mediate antiviral protection, tetracycline was used to decrease the abundance of the high-density, protective Wolbachia strain wAu prior to viral challenge. Antiviral protection was lost when the density of the protective Wolbachia strain was decreased to an abundance similar to that of nonprotective Wolbachia strains. We determined the Wolbachia density and distribution in tissues of the same five fly-Wolbachia combinations as used previously. The Wolbachia density within the head, gut, and Malpighian tubules correlated with the ability to mediate antiviral protection. These findings may facilitate the development of Wolbachia biological control strategies and help to predict host-Wolbachia pairings that may interfere with virus-induced pathology.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Biological Sciences Publications
Institute for Molecular Bioscience - Publications
 
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Created: Tue, 13 Nov 2012, 11:01:50 EST by Gail Walter on behalf of School of Biological Sciences