Comparative genomics of the Staphylococcus intermedius group of animal pathogens

Ben Zakour, Nouri L., Beatson, Scott A., van den Broek, Adri H. M., Thoday, Keith L. and Fitzgerald, J. Ross (2012) Comparative genomics of the Staphylococcus intermedius group of animal pathogens. Frontiers in Cellular and Infection Microbiology, 2 44.1-44.15. doi:10.3389/fcimb.2012.00044

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Author Ben Zakour, Nouri L.
Beatson, Scott A.
van den Broek, Adri H. M.
Thoday, Keith L.
Fitzgerald, J. Ross
Title Comparative genomics of the Staphylococcus intermedius group of animal pathogens
Formatted title
Comparative genomics of the Staphylococcus intermedius group of animal pathogens
Journal name Frontiers in Cellular and Infection Microbiology   Check publisher's open access policy
ISSN 2235-2988
Publication date 2012-04
Sub-type Article (original research)
DOI 10.3389/fcimb.2012.00044
Open Access Status DOI
Volume 2
Start page 44.1
End page 44.15
Total pages 15
Place of publication Lausanne, Switzerland
Publisher Frontiers Research Foundation
Collection year 2013
Language eng
Formatted abstract
The Staphylococcus intermedius group consists of three closely related coagulase-positive bacterial species including S. intermedius, Staphylococcus pseudintermedius, and Staphylococcus delphini. S. pseudintermedius is a major skin pathogen of dogs, which occasionally causes severe zoonotic infections of humans. S. delphini has been isolated from an array of different animals including horses, mink, and pigeons, whereas S. intermedius has been isolated only from pigeons to date. Here we provide a detailed analysis of the S. pseudintermedius whole genome sequence in comparison to high quality draft S. intermedius and S. delphini genomes, and to other sequenced staphylococcal species. The core genome of the SIG was highly conserved with average nucleotide identity (ANI) between the three species of 93.61%, which is very close to the threshold of species delineation (95% ANI), highlighting the close-relatedness of the SIG species. However, considerable variation was identified in the content of mobile genetic elements, cell wall-associated proteins, and iron and sugar transporters, reflecting the distinct ecological niches inhabited. Of note, S. pseudintermedius ED99 contained a clustered regularly interspaced short palindromic repeat locus of the Nmeni subtype and S. intermedius contained both Nmeni and Mtube subtypes. In contrast to S. intermedius and S. delphini and most other staphylococci examined to date, S. pseudintermedius contained at least nine predicted reverse transcriptase Group II introns. Furthermore, S. pseudintermedius ED99 encoded several transposons which were largely responsible for its multi-resistant phenotype. Overall, the study highlights extensive differences in accessory genome content between closely related staphylococcal species inhabiting distinct host niches, providing new avenues for research into pathogenesis and bacterial host-adaptation.
Keyword Staphylococcus
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article # 44

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Chemistry and Molecular Biosciences
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Created: Sun, 11 Nov 2012, 11:20:59 EST by Dr Scott Beatson on behalf of School of Chemistry & Molecular Biosciences