An insect trypsin-like serine protease as a target of miRNA: utilization of miRNA mimics and inhibitors by oral feeding

Jayachandran, Balanchandran, Hussain, Mazhar and Asgari, Sassan (2013) An insect trypsin-like serine protease as a target of miRNA: utilization of miRNA mimics and inhibitors by oral feeding. Insect Biochemistry and Molecular Biology, 43 4: 398-406.


Author Jayachandran, Balanchandran
Hussain, Mazhar
Asgari, Sassan
Title An insect trypsin-like serine protease as a target of miRNA: utilization of miRNA mimics and inhibitors by oral feeding
Journal name Insect Biochemistry and Molecular Biology  (ERA 2012 Listed)    (ERA 2010 Rank A)   Check publisher's open access policy
Publication date 2013-04
Sub-type Article
Year available 2012
DOI 10.1016/j.ibmb.2012.10.004
Volume number 43
Issue number 4
ISSN 0965-1748; 1879-0240
Start page 398
End page 406
Total pages 9
Place of publication Kidlington, Oxford, United Kingdom
Publisher Pergamon
Collection year 2013
Language eng
Formatted abstract MicroRNAs (miRNAs) are non-coding small RNAs (18e25 nt) that play crucial roles in various biological processes, including development, as gene regulators. In this study, we identified a miRNA, har-miR-2002b, that is specifically expressed during larval stages of Helicoverpa armigera (cotton bollworm). For the first time, we explored the possibility of utilizing synthetic inhibitor and mimic of a miRNA by oral feeding and confirmed that the inhibitor and mimic can successfully inhibit/oversupply har-miR-2002b in H. armigera larvae. Bioassays by oral feeding indicated 70% reduction in fecundity and 40% larval mortality in the presence of har-miR-2002b mimic. In addition, a large proportion of those larvae that made it to the pupal stage were deformed and only few moths emerged from them. This implied an important role of har-miR-2002b in larval development and adult fecundity. A trypsin-like serine protease (Ha-TLP) was identified as a target of har-miR-2002b. In vivo and in vitro studies revealed that in the presence of the miRNA mimic Ha-TLP transcript/protein levels and enzyme activity were significantly reduced. In contrast, inhibition of har-miR-2002b led to significant up-regulation of Ha-TLP transcript levels. This is the first study which shows the potential of utilization of miRNA mimics and inhibitors in pest control by targeting specific insect genes.
Keyword MicroRNA
Mimic
Inhibitor
Trypsin-like serine protease
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online: 26 October 2012.

Document type: Journal Article
Sub-type: Article
Collections: School of Biological Sciences Publications
Official 2013 Collection
 
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Created: Wed, 07 Nov 2012, 14:45:30 EST by Gail Walter on behalf of School of Biological Sciences