Reduction in the size of layered double hydroxide nanoparticles enhances the efficiency of siRNA delivery

Chen, Min, Helen Cooper, Zhou, Ji Zhi, Bartlett, Perry F. and Xu, Zhi Ping (2013) Reduction in the size of layered double hydroxide nanoparticles enhances the efficiency of siRNA delivery. Journal of Colloid and Interface Science, 390 1: 275-281. doi:10.1016/j.jcis.2012.09.033

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Author Chen, Min
Helen Cooper
Zhou, Ji Zhi
Bartlett, Perry F.
Xu, Zhi Ping
Title Reduction in the size of layered double hydroxide nanoparticles enhances the efficiency of siRNA delivery
Journal name Journal of Colloid and Interface Science   Check publisher's open access policy
ISSN 0021-9797
1095-7103
Publication date 2013-01-15
Year available 2012
Sub-type Article (original research)
DOI 10.1016/j.jcis.2012.09.033
Open Access Status File (Author Post-print)
Volume 390
Issue 1
Start page 275
End page 281
Total pages 7
Place of publication Maryland Heights, MO, United States
Publisher Academic Press
Collection year 2013
Language eng
Abstract Small interfering RNAs (siRNAs) are a potentially powerful new class of pharmaceutical drugs for many disease. However, the delivery of unprotected siRNAs is ineffective due to their susceptibility to degradation by ubiquitous nucleases under physiological conditions. Layered double hydroxide nanoparticles (LDHs) have been found to be efficient carriers of anionic drugs and nucleic acids. Our previous research has shown that LDHs (with the Z-average particle size of approximately 110 nm) can mediate siRNA delivery in mammalian cells, resulting in gene silencing. However, short double-stranded nucleic acids are mostly adsorbed onto the external surface and not well protected by LDHs. In order to enhance the intercalation of siRNA into the LDH interlayer and the efficiency of subsequent siRNA delivery, we prepared smaller LDHs (with the Z-average particle size of approximately 45 nm) with an engineered non-aqueous method. We demonstrate here that dsDNA/siRNA is more effectively intercalated into these small LDH nanoparticles, more dsDNA/siRNA is transfected into HEK 293T cells, and more efficient silencing of the target gene is achieved using smaller LDHs. Thus, smaller LDH particles have greater potential as a delivery system for the application of RNA interference.
Keyword Layered double hydroxide nanoparticles
RNA interference
Gene delivery
Cellular uptake
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 26 September 2012

 
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Created: Tue, 06 Nov 2012, 13:16:41 EST by Debra McMurtrie on behalf of Queensland Brain Institute