Gene expression analysis of pig macrophages reveals similarities to humans

Kapetanovic, R., Fairbairn, L., Sester, D. P., Archibald, A. L. and Hume, D. A. (2012). Gene expression analysis of pig macrophages reveals similarities to humans. In: European Congress of Immunology: Poster sessions. European Congress of Immunology, Glasgow, Scotland, (268-268). 5-8 September 2012. doi:10.1111/imm.12002

Author Kapetanovic, R.
Fairbairn, L.
Sester, D. P.
Archibald, A. L.
Hume, D. A.
Title of paper Gene expression analysis of pig macrophages reveals similarities to humans
Conference name European Congress of Immunology
Conference location Glasgow, Scotland
Conference dates 5-8 September 2012
Proceedings title European Congress of Immunology: Poster sessions   Check publisher's open access policy
Journal name Immunology   Check publisher's open access policy
Place of Publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing
Publication Year 2012
Sub-type Published abstract
DOI 10.1111/imm.12002
ISSN 0019-2805
Volume 137
Issue s1
Start page 268
End page 268
Total pages 1
Language eng
Formatted Abstract/Summary
Purpose/Objective: The laboratory mouse is an imperfect model in
which to study human innate immunity. The larger size of the pig, and
closer evolutionary distance to humans, offer several advantages. In
particular, it is more straightforward to access large numbers of lung
macrophages. In this study, we compared the gene expression profiles
of macrophages from different breeds and compartments; alveolar
(AM), bone-marrow-derived, monocyte subsets and monocyte-derived
Materials and methods: We isolated a large number of mononuclear
cells from the lung, bone-marrow and blood of 25 pigs from five
breeds. Phagocytosis, TNF production and the expression of macrophage
markers were characterised. We used a newly-generated and
annotated pig expression array to characterise gene expression and the
response to lipopolysaccharide.
Results: Isolated macrophage populations from pigs resemble those of
humans. All type of macrophages expresses CD16, the LPS co-receptor
CD14, CD172a. CD163 expression defined a subset of monocytes, and
was expressed inversely with CD14. It was retained on alveolar
macrophages (AM). Alveolar macrophages had a specific gene
expression profile that included high levels of many C type lectins.
Like peripheral blood monocytes, AM comprised two two subpopulations
that differed in adherence, LPS response, phagocytosis and
expression of CD163. CD14++ monocytes resembled CD14++ human
monocytes in the expression profile. Human and pig macrophages also
shared expression of LPS-inducible genes (STAT4, IDO, CCL20,
Cyp27B1) that are not induced in mouse macrophages, and failed to
induce iNOS. Pig breeds showed no great differences in response to
LPS. The few genes differentially expressed included cytochrome
CYP3A29, the metalloproteinase MMP1, STEAP4 and the N-Myc
interactor NMI.
Conclusions: We have isolated and characterised the gene expression
profiles of pig macrophages in multiple differentiation and activation
states. The data support the use of the pig as a model of innate
immunity that more closely resembles
Q-Index Code CX
Q-Index Status Provisional Code
Institutional Status UQ

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Created: Fri, 26 Oct 2012, 15:43:10 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences