Prevalence of koala retrovirus in geographically diverse populations in Australia

Simmons, G. S., Young, P. R., Hanger, J. J., Jones, K., Clarke, D., McKee, J. J. and Meers, J. (2012) Prevalence of koala retrovirus in geographically diverse populations in Australia. Australian Veterinary Journal, 90 10: 404-409. doi:10.1111/j.1751-0813.2012.00964.x


Author Simmons, G. S.
Young, P. R.
Hanger, J. J.
Jones, K.
Clarke, D.
McKee, J. J.
Meers, J.
Title Prevalence of koala retrovirus in geographically diverse populations in Australia
Journal name Australian Veterinary Journal   Check publisher's open access policy
ISSN 0005-0423
1751-0813
Publication date 2012-10
Sub-type Article (original research)
DOI 10.1111/j.1751-0813.2012.00964.x
Volume 90
Issue 10
Start page 404
End page 409
Total pages 6
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing
Collection year 2013
Language eng
Formatted abstract
Objective To determine the prevalence of koala retrovirus (KoRV) in selected koala populations and to estimate proviral copy number in a subset of koalas.
Methods Blood or tissue samples from 708 koalas in Queensland, New South Wales, Victoria and South Australia were tested for KoRV pol provirus gene using standard polymerase chain reaction (PCR), nested PCR and real-time PCR (qPCR).
Results Prevalence of KoRV provirus-positive koalas was 100% in four regions of Queensland and New South Wales, 72.2% in mainland Victoria, 26.6% on four Victorian islands and 14.8% on Kangaroo Island, South Australia. Estimated proviral copy number per cell in four groups of koalas from Queensland and Victoria showed marked variation, ranging from a mean of 165 copies per cell in the Queensland group to 1.29 × 10−4 copies per cell in one group of Victorian koalas.
Conclusions The higher prevalence of KoRV-positive koalas in the north of Australia and high proviral loads in Queensland koalas may indicate KoRV entered and became endogenous in the north and is spreading southwards. It is also possible there are genetic differences between koalas in northern and southern Australia that affect susceptibility to KoRV infection or endogenisation, or that environmental factors affecting transmission in northern states are absent or uncommon in southern regions. Although further studies are required, the finding of proviral copy numbers orders of magnitude lower than what would be expected for the presence of a single copy in every cell for many Victorian animals suggests that KoRV is not endogenous in these animals and likely reflects ongoing exogenous infection.
Keyword Epidemiology
Koalas
Molecular virology
Wildlife
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Chemistry and Molecular Biosciences
School of Veterinary Science Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 31 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 34 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Fri, 26 Oct 2012, 14:37:27 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences