eLCR: electrochemical detection of single DNA base changes via ligase chain reaction

Wee, Eugene J. H., Shiddiky, Muhammad J. A., Brown, Melissa A. and Trau, Matt (2012) eLCR: electrochemical detection of single DNA base changes via ligase chain reaction. Chemical Communications, 48 98: 1214-1216. doi:10.1039/C2CC35841G


Author Wee, Eugene J. H.
Shiddiky, Muhammad J. A.
Brown, Melissa A.
Trau, Matt
Title eLCR: electrochemical detection of single DNA base changes via ligase chain reaction
Journal name Chemical Communications   Check publisher's open access policy
ISSN 1359-7345
1364-548X
Publication date 2012-10-24
Sub-type Article (original research)
DOI 10.1039/C2CC35841G
Volume 48
Issue 98
Start page 1214
End page 1216
Total pages 3
Place of publication Cambridge, United Kingdom
Publisher RSC
Collection year 2013
Language eng
Abstract Simple, inexpensive and wide-scaled analysis of single DNA base changes (‘point mutations’ which include SNP’s, somatic mutations and epigenetic changes) is one of the holy grail’s of point-of-care diagnostics. Herein, we present eLCR: a simple methodology that fuses Ligase Chain Reaction (LCR) with electrochemical detection based on DNA-mediated charge transport. LCR generates long and short “knife” motifs representing the presence or absence of single DNA base changes, which are then detected electrochemically by either methylene blue intercalation or horseradish peroxidase labelling.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes First published online: 24 October 2012.

 
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Created: Thu, 25 Oct 2012, 11:33:16 EST by Dr Muhammad J. A. Shiddiky on behalf of Aust Institute for Bioengineering & Nanotechnology