Developmental and functional evidence of nuclear immaturity in prepubertal oocytes

Ptak, Grazyna, Matsukawa, Kazutsugu, Palmieri, Chiara, Della Salda, Leonardo, Scapolo, Pier Augusto and Loi, Pasqualino (2006) Developmental and functional evidence of nuclear immaturity in prepubertal oocytes. Human Reproduction, 21 9: 2228-2237. doi:10.1093/humrep/del184


Author Ptak, Grazyna
Matsukawa, Kazutsugu
Palmieri, Chiara
Della Salda, Leonardo
Scapolo, Pier Augusto
Loi, Pasqualino
Title Developmental and functional evidence of nuclear immaturity in prepubertal oocytes
Journal name Human Reproduction   Check publisher's open access policy
ISSN 0268-1161
1460-2350
Publication date 2006-09
Sub-type Article (original research)
DOI 10.1093/humrep/del184
Volume 21
Issue 9
Start page 2228
End page 2237
Total pages 10
Place of publication Oxford, United Kingdom
Publisher Oxford University Press
Language eng
Formatted abstract
BACKGROUND: The nuclear compartment has been proposed as responsible for the developmental arrest of prepubertal mouse oocytes while the studies on prepubertal sheep and cow oocyte model suggested the cytoplasm immaturity accounts for this failure. METHODS: The apparent disagreement on the causes of developmental defects between these two species prompted us to study: (i) follicular and oocyte growth allometry in lambs, (ii) oocyte compartment (nucleus versus cytoplasm) responsible for developmental failure by nucleus exchange between lamb and adult sheep oocytes, (iii) nucleolar features of prepubertal oocytes by ultrastructural observation and (iv) in vivo developmental survey of prepubertally derived embryos. RESULTS: The oocyte growth inside the follicle is asynchronous during prepuberty. The nuclear transfer revealed that the lamb nucleus was responsible for developmental failure. Immature fibrillogranular structure of the nucleolus has been revealed in small lamb oocytes and also in a few adult-size lamb oocytes. Studies in vivo revealed a high occurrence of developmental arrest of prepubertal derived fetuses, which we have attributed to the low genome-wide methylation detected in prepubertal oocytes. CONCLUSIONS: Our studies have indicated incomplete nuclear maturation of prepubertal gamete. The implication of this finding suggests caution when the strategy of rescue of prepubertal oocytes for assisted fertilization is considered such as in the case of therapeutic treatment which precludes the maintenance of fertility of sexually immature patients.
Keyword Developmental arrest
Methylation
Nuclear immaturity
Oocyte
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Veterinary Science Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 17 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 17 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Fri, 19 Oct 2012, 17:16:51 EST by Chiara Palmieri on behalf of School of Veterinary Science