Placental abnormalities associated with post-natal mortality in sheep somatic cell clones

Loi, Pasqualino, Clinton, Michael, Vackova, Irena, Fulka, Josef, Feil, Robert, Palmieri, Chiara, Della Salda, Leonardo and Ptak, Grazyna (2006) Placental abnormalities associated with post-natal mortality in sheep somatic cell clones. Theriogenology, 65 6: 1110-1121. doi:10.1016/j.theriogenology.2005.07.016


Author Loi, Pasqualino
Clinton, Michael
Vackova, Irena
Fulka, Josef
Feil, Robert
Palmieri, Chiara
Della Salda, Leonardo
Ptak, Grazyna
Title Placental abnormalities associated with post-natal mortality in sheep somatic cell clones
Journal name Theriogenology   Check publisher's open access policy
ISSN 0093-691X
1879-3231
Publication date 2006-04
Sub-type Article (original research)
DOI 10.1016/j.theriogenology.2005.07.016
Volume 65
Issue 6
Start page 1110
End page 1121
Total pages 12
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Language eng
Formatted abstract
We report on cloning experiments designed to explore the causes of peri- and post-natal mortality of cloned lambs. A total of 93 blastocysts obtained by nuclear transfer of somatic cells (granulosa cells) were transferred into 41 recipient ewes, and pregnancies were monitored by ultrasound scanning. In vitro derived, fertilized embryos (IVF, n = 123) were also transferred to assess oocyte competence, and naturally mated ewes (n = 120) were analysed as well. Cloned embryos developed to the blastocyst stage and implanted at the same rate as IVF embryos. After day 30 of gestation, however, dramatic losses occurred, and only 12 out of 93 (13%) clones reached full-term development, compared to 51 out of 123 (41.6%) lambs born from the IVF control embryos. Three full-term lamb clones were delivered stillborn, as a result of placental degeneration. A further five clone recipients developed hydroallantois. Their lambs died within 24 h following delivery by caesarian section, and displayed degenerative lesions in liver and kidney resulting from the severe hydroallantois. One set of twins was delivered by assisted parturition at day 150, but died 24 h later due to respiratory distress syndrome. The remaining two clone recipients underwent caesarian section, and the corresponding two lambs displayed signs of respiratory dysfunction and died at approximately 1 month of age due to a bacterial complication. Blood samples collected from the cloned lambs after birth revealed a wide range of abnormalities indicative of kidney and liver dysfunction. Macroscopical and histopathological examination of the placentae revealed a marked reduction in vascularization, particularly at the apex of the villous processes, as well as a loss of differentiation of the trophoblastic epithelium. Our results strongly suggest that post-mortality in cloned lambs is mainly caused by placental abnormalities.
Keyword Cloning
Post-natal mortality
Placental abnormalities
Lambs
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Veterinary Science Publications
 
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Created: Fri, 19 Oct 2012, 17:15:48 EST by Chiara Palmieri on behalf of School of Veterinary Science