Placental abnormalities in ovine somatic cell clones at term: a light and electron microscopic investigation

Palmieri, C., Loi, P., Reynolds, L. P., Ptak, G. and Della Salda, L. (2007) Placental abnormalities in ovine somatic cell clones at term: a light and electron microscopic investigation. Placenta, 28 5-6: 577-584. doi:10.1016/j.placenta.2006.08.003


Author Palmieri, C.
Loi, P.
Reynolds, L. P.
Ptak, G.
Della Salda, L.
Title Placental abnormalities in ovine somatic cell clones at term: a light and electron microscopic investigation
Journal name Placenta   Check publisher's open access policy
ISSN 0143-4004
1532-3102
Publication date 2007-05
Sub-type Article (original research)
DOI 10.1016/j.placenta.2006.08.003
Volume 28
Issue 5-6
Start page 577
End page 584
Total pages 8
Place of publication Oxford, United Kingdom
Publisher Elsevier
Language eng
Abstract To investigate the reasons for fetal losses after somatic cell nuclear transfer, an immunohistochemical and ultrastructural analysis of cloned placentae was performed. The main features observed were a marked reduction of villous vascularization, hypoplasia of trophoblastic epithelium, lack of binucleate cells, immaturity of placental vessels and reduced vasculogenesis. By means of transmission electron microscopy (TEM), a diffuse thickening and lamination of subtrophoblastic basement membrane (SBM) were noted in cloned placentae. These results led us to hypothesize, through an autoamplification model, that the abnormal vascularization, the ischaemia and the low development of an high specialized trophoblastic epithelium were the primary causes of the fetal loss occurring after somatic cells nuclear transfer.
Keyword Somatic cell nuclear transfer
Placenta
Immunohistochemistry
Electron microscopy
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Veterinary Science Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 16 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 15 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Fri, 19 Oct 2012, 16:42:00 EST by Chiara Palmieri on behalf of School of Veterinary Science