Heat shock protein expression in canine osteosarcoma

Romanucci, Mariarita, D'Amato, Giuliana, Malatesta, Daniela, Bongiovanni, Laura, Palmieri, Chiara, Ciccarelli, Andrea, Buracco, Paolo, Morello, Emanuela, Maniscalco, Lorella, De Maria, Raffaella, Martano, Marina and Della Salda, Leonardo (2012) Heat shock protein expression in canine osteosarcoma. Cell Stress and Chaperones, 17 1: 131-138. doi:10.1007/s12192-011-0294-2

Author Romanucci, Mariarita
D'Amato, Giuliana
Malatesta, Daniela
Bongiovanni, Laura
Palmieri, Chiara
Ciccarelli, Andrea
Buracco, Paolo
Morello, Emanuela
Maniscalco, Lorella
De Maria, Raffaella
Martano, Marina
Della Salda, Leonardo
Title Heat shock protein expression in canine osteosarcoma
Journal name Cell Stress and Chaperones   Check publisher's open access policy
ISSN 1355-8145
Publication date 2012-01
Year available 2011
Sub-type Article (original research)
DOI 10.1007/s12192-011-0294-2
Volume 17
Issue 1
Start page 131
End page 138
Total pages 8
Place of publication Dordrecht, Netherlands
Publisher Springer
Collection year 2013
Language eng
Formatted abstract
Abnormal levels of heat shock proteins have been observed in a number of human neoplasms and demonstrate prognostic, predictive and therapeutic implications. Since osteosarcoma (OSA) in dogs provides an important model for the same disease in humans, the aim of this study was to evaluate the immunohistochemical expression of Hsp27, Hsp72, Hsp73 and Hsp90 in 18 samples of canine appendicular OSA, in relation to histological grade and overall survival (OS), in order to investigate their potential prognostic, predictive and/or therapeutic value. A semiquantitative method was used for the analysis of the results. Hsp27, Hsp73 and Hsp90 showed a variably intense, cytoplasmic and nuclear immunoreactivity that was not associated with histological type or grade. On the other hand, a high percentage of Hsp72 immunostaining was significantly associated with grade III (P < 0.01) and a lack of immunolabelling was significantly correlated to a longer OS (P = 0.006). Neoplastic emboli were occasionally positive for Hsp27, faintly immunoreactive for Hsp72 and intensely immunolabelled by Hsp73 and Hsp90. In conclusion, absence of Hsp72 immunosignal appears to be associated with a favourable prognosis whilst the widespread Hsp90 immunoreactivity detected in all tumour cases as well as in neoplastic emboli, suggests this protein could be targeted in the therapy of canine OSA, and likewise in its human counterpart.
Keyword Heat shock proteins
Animal model
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ
Additional Notes Published online: 21 October 2011

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Veterinary Science Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 5 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 5 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Thu, 18 Oct 2012, 14:13:39 EST by Chiara Palmieri on behalf of School of Veterinary Science