A short exposure to polychlorinated biphenyls deregulates cellular autophagy in mammalian blastocyst in vitro

Ptak, Grazyna, Zacchini, Federica, Czernik, Marta, Fidanza, Antonella, Palmieri, Chiara, Della Salda, Leonardo, Scapolo, Pier Augusto and Loi, Pasqualino (2012) A short exposure to polychlorinated biphenyls deregulates cellular autophagy in mammalian blastocyst in vitro. Human Reproduction, 27 4: 1034-1042.


Author Ptak, Grazyna
Zacchini, Federica
Czernik, Marta
Fidanza, Antonella
Palmieri, Chiara
Della Salda, Leonardo
Scapolo, Pier Augusto
Loi, Pasqualino
Title A short exposure to polychlorinated biphenyls deregulates cellular autophagy in mammalian blastocyst in vitro
Formatted title A short exposure to polychlorinated biphenyls deregulates cellular autophagy in mammalian blastocyst in vitro
Journal name Human Reproduction  (ERA 2012 Listed)    (ERA 2010 Rank A)   Check publisher's open access policy
Publication date 2012-04
Sub-type Article
DOI 10.1093/humrep/der454
Volume number 27
Issue number 4
ISSN 0268-1161; 1460-2350
Start page 1034
End page 1042
Total pages 9
Place of publication Oxford, United Kingdom
Publisher Oxford University Press
Collection year 2013
Language eng
Formatted abstract BACKGROUND: Polychlorinated biphenyls (PCBs) are common environmental contaminants that represent an important risk factor of reproductive disorders in chronically exposed human populations. However, it is not known whether a short accidental exposure of embryos to PCBs before implantation might influence their further development and whether the effect might be reversible.
METHODS AND RESULTS: To this aim, in vitro-matured sheep blastocysts were incubated with 2 or 4 µg/ml Aroclor 1254 (A1254), a mixture of 60 PCB congeners for 48h after which blastocyst proliferation and ability for outgrowth in vitro were assessed. Blastocysts exposed to A1254 showed: (i) reduced proliferation and cell number (particularly in the inner cell mass compartment); (ii) accumulation of vacuoles and lipid droplets, diffused mitochondrial damage and up-regulation of autophagy markers (ATG6 and LC3), all signs indicative of deregulated autophagy, and (iii) massive cell death. Although exposed embryos resumed growth following A1254 removal, their subsequent development remained severely perturbed.
CONCLUSIONS: These findings indicate that short exposure of blastocysts to PCBs leads to its damage characterized by deregulated autophagy and subsequent cell death.
Keyword PCBs
Blastocyst
Apoptosis
Autophagy
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article
Collections: Non HERDC
School of Veterinary Science Publications
 
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Created: Thu, 18 Oct 2012, 13:58:59 EST by Chiara Palmieri on behalf of School of Veterinary Science