Immobilization of carbonic anhydrase on mesoporous aluminosilicate for carbonation reaction

Wanjari, Snehal, Prabhu, Chandan, Satyanarayana, T., Vinu, Ajayan and Rayalu, Sadhana (2012) Immobilization of carbonic anhydrase on mesoporous aluminosilicate for carbonation reaction. Microporous and Mesoporous Materials, 160 151-158. doi:10.1016/j.micromeso.2012.04.005

Author Wanjari, Snehal
Prabhu, Chandan
Satyanarayana, T.
Vinu, Ajayan
Rayalu, Sadhana
Title Immobilization of carbonic anhydrase on mesoporous aluminosilicate for carbonation reaction
Journal name Microporous and Mesoporous Materials   Check publisher's open access policy
ISSN 1387-1811
Publication date 2012-09-15
Sub-type Article (original research)
DOI 10.1016/j.micromeso.2012.04.005
Volume 160
Start page 151
End page 158
Total pages 8
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2013
Language eng
Formatted abstract
Mesoporous aluminosilicates synthesized using a template of Pluronic F127 with high surface area and pore diameter appears to be tailor made for enzyme adsorption. Carbonic anhydrase (CA) with diameter of 35 Å has been immobilized in these crystalline, ordered mesoporous materials and tested for mechanistic and kinetic aspects using p-NPA assay. Km, Vmax and Kcat of the CAImAlK was observed to be 0.158 mM, 2.307 μmol/min/ml and 1.9 s−1, respectively compared to Km, Vmax and Kcat of 0.876 mM, 0.936 μmol/min/ml and 2.3 s−1, respectively for free CA. Storage stability test was conducted up to 25 days and it was observed that the HLP at 25 °C improved by a factor of 1.6 for CAImAlK (600 h) compared to 360 h for free CA. Proof of concept has been established for carbonation reaction. The CO2 sequestration capacity in terms of conversion of CO2 to calcium carbonate was quantified by gas chromatography (GC). The CO2 sequestration capacity of CAImAlK was determined to be 16.14 mg of CaCO3/mg of CA compared to 33.08 mg of CaCO3/mg of CA for free CA.
Keyword CO2 sequestration
Mesoporous aluminosilicates
Carbonic anhydrase
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
Australian Institute for Bioengineering and Nanotechnology Publications
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