Biomaterial immobilization in nanoporous carbon molecular sieves: influence of solution pH, pore volume, and pore diameter

Vinu, Ajayan, Miyahara, Masahiko and Ariga, Katsuhiko (2005) Biomaterial immobilization in nanoporous carbon molecular sieves: influence of solution pH, pore volume, and pore diameter. Journal of Physical Chemistry B, 109 13: 6436-6441. doi:10.1021/jp050454o


Author Vinu, Ajayan
Miyahara, Masahiko
Ariga, Katsuhiko
Title Biomaterial immobilization in nanoporous carbon molecular sieves: influence of solution pH, pore volume, and pore diameter
Journal name Journal of Physical Chemistry B   Check publisher's open access policy
ISSN 1520-6106
1520-5207
Publication date 2005-04
Sub-type Article (original research)
DOI 10.1021/jp050454o
Volume 109
Issue 13
Start page 6436
End page 6441
Total pages 6
Place of publication Washington, DC, United States
Publisher American Chemical Society
Language eng
Formatted abstract
The adsorption of lysozyme (Lz) onto nanoporous carbon molecular sieves with various pore diameters has been studied at different solution pH values. All the adsorption isotherms have successfully been correlated by the Langmuir equation. The amount of adsorbed Lz depends on the solution pH as well as on the specific pore volume and pore diameter of the adsorbents. The maximum adsorption was observed near the isoelectric point of the Lz (pI ≈ 11), suggesting that suppression of electric repulsion between the enzymes plays an important role in the adsorption process. Moreover, the amount adsorbed depends on the pore size and pore volume of the nanoporous carbon adsorbents, indicating that the Lz molecules are adsorbed inside the mesopores. CMK-3-150 shows a larger amount of Lz adsorption as compared to CMK-3. The increased Lz adsorption capacity of CMK-3-150 may be due to the larger pore volume and pore diameter as compared to that of CMK-3. The unaltered structural order of the nanoporous adsorbents after the adsorption has been confirmed by the physicochemical characterization techniques such as XRD and N2 adsorption. In addition, FT-IR spectroscopic studies confirm that the Lz used in this study is stable even after the adsorption on the nanoporous carbon. These results indicate that nanoporous carbon has superior water stability and thus is a more appropriate adsorbent for biomaterials than nanoporous silica.
Keyword Adsorption
Lysozyme
Molecular sieves
Electric repulsion
Enzymes
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
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