Adsorption of cytochrome c on mesoporous molecular sieves: Influence of pH, pore diameter, and aluminum incorporation

Vinu, A., Murugesan, V., Tangermann, Oliver and Hartmann, Martin (2004) Adsorption of cytochrome c on mesoporous molecular sieves: Influence of pH, pore diameter, and aluminum incorporation. Chemistry of Materials, 16 16: 3056-3065. doi:10.1021/cm049718u


Author Vinu, A.
Murugesan, V.
Tangermann, Oliver
Hartmann, Martin
Title Adsorption of cytochrome c on mesoporous molecular sieves: Influence of pH, pore diameter, and aluminum incorporation
Journal name Chemistry of Materials   Check publisher's open access policy
ISSN 0897-4756
1520-5002
Publication date 2004-08
Sub-type Article (original research)
DOI 10.1021/cm049718u
Volume 16
Issue 16
Start page 3056
End page 3065
Total pages 10
Place of publication Washington, DC, United States
Publisher American Chemical Society
Language eng
Formatted abstract
The adsorption of cytochrome c onto different mesoporous molecular sieves (C12-MCM-41, C16-MCM-41 and SBA-15) is studied at different solution pHs. Adsorption isotherms were recorded up to final solution concentrations of ca. 250 μmol/L and were found to be of the pseudo-Langmuir type. Cytochrome c (cyt c) adsorption was observed to be pH-dependent with maximum adsorption near the isoelectric point of the protein. SBA-15 showed a larger amount of cyt c adsorption as compared to MCM-41. The increased cyt c adsorption capacity may be due to the larger pore volume and pore diameter as compared to C12- and C16-MCM-41. It has been discovered that the amount of cyt c adsorption can be increased by the introduction of aluminum into the pure silica materials. The observed increase is most likely a consequence of the strong electrostatic interaction between the negative charges on the aluminum sites and the positively charged amino acid residues of cyt c. Furthermore, the rate of cyt c adsorption has been studied and no significant differences in adsorption rate were found for the mesoporous materials with different pore diameters between 3 and 9 nm. While the adsorption capacity is reduced upon bead formation (due to the reduction in specific pore volume), the rate of adsorption is mainly unchanged.
Keyword Adsorption
Cytochrome
Isotherms
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 253 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 259 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 16 Oct 2012, 11:18:28 EST by System User on behalf of Aust Institute for Bioengineering & Nanotechnology