Immobilization of lysozyme onto pore-engineered mesoporous AISBA-15

Miyahara, Masahiko, Vinu, Ajayan and Ariga, Katsuhiko (2006) Immobilization of lysozyme onto pore-engineered mesoporous AISBA-15. Journal of Nanoscience and Nanotechnology, 6 6: 1765-1771. doi:10.1166/jnn.2006.221

Author Miyahara, Masahiko
Vinu, Ajayan
Ariga, Katsuhiko
Title Immobilization of lysozyme onto pore-engineered mesoporous AISBA-15
Journal name Journal of Nanoscience and Nanotechnology   Check publisher's open access policy
ISSN 1533-4880
Publication date 2006-06
Sub-type Article (original research)
DOI 10.1166/jnn.2006.221
Volume 6
Issue 6
Start page 1765
End page 1771
Total pages 7
Place of publication Valencia, CA United States
Publisher American Scientific Publishers
Collection year 2006
Language eng
Formatted abstract
In this research, hydrothermally stable mesoporous AISBA-15 materials have been used as adsorbents for systematic research on the lysozyme adsorption. Stability of the AISBA-15 adsorbents and the lysozyme molecules after the adsorption experiments for several days in aqueous solutions were confirmed by X-ray diffraction (XRD) measurement and FT-IR spectroscopy, respectively. The amount of the lysozyme adsorption can easily be controlled by the pore diameter and pore volume the mesoporous adsorbent, but an unreasonable effect of the surface area on the protein adsorption capacity was observed. The results of the effect of the pore diameter on the lysozyme adsorption suggest that the adsorption might partially be influenced by kinetically favorable edge-on type orientation on the confined mesopore. However, the final adsorption amount of the lysozyme can be well regenerated by models based on the side-on adsorption in dense packing. The present research also confirms the importance of appropriate "pore-engineering" for immobilization of bio-function on mesoporous materials.
Keyword Mesoporous silica
Pore Volume
Pore Diameter
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
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